Skip Navigation


JXB Advance Access originally published online on September 10, 2004
Journal of Experimental Botany 2004 55(406):2169-2177; doi:10.1093/jxb/erh234
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrowOA All Versions of this Article:
55/406/2169    most recent
erh234v1
Right arrow E-letters: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when E-letters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (3)
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Parfitt, D.
Right arrow Articles by Francis, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Parfitt, D.
Right arrow Articles by Francis, D.
Agricola
Right arrow Articles by Parfitt, D.
Right arrow Articles by Francis, D.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Journal of Experimental Botany, Vol. 55, No. 406, © Society for Experimental Biology 2004; all rights reserved

RESEARCH PAPER

Differential expression of putative floral genes in Pharbitis nil shoot apices cultured on glucose compared with sucrose

D. Parfitt1,2, R. J. Herbert1, H. J. Rogers2 and D. Francis2,*

1Department of Applied Sciences, Archaeology and Geography, University College Worcester, Henwick Grove, Worcester WR2 6AJ, UK
2School of Biosciences, Cardiff University, PO Box 915, Cardiff CF10 3TL, UK

* To whom correspondence should be addressed. Fax: +44 29 2087 4305. E-mail: francisd{at}cf.ac.uk

If, following an inductive treatment of 2 d of continuous darkness, shoot apices of Pharbitis nil are cultured 1 d later on White's medium supplemented with 2% sucrose, they cannot form carpels, but they can if they are cultured on 2% glucose. It was hypothesized that the differential effect of these sugars was because of differential expression of carpel-specific genes. Partial cDNA homologues to the Arabidopsis genes, LEAFY (PnLFY), AGAMOUS (PnAG1/2), and CRABS CLAWS (PnCRC1/2) were cloned. PnLFY was expressed in the shoot apex 1 d following the start of induction and remained higher than in non-induced apices for a further 6 d before exhibiting a major peak of expression on day 7. Peaks of expression of PnAG1 and PnAG2 spanned days 7–11, coinciding with the appearance of stamens and then carpels. The Pharbitis ‘PnCRC2’ showed greatest homology to Arabidopsis YABBY2 (PnYABBY). Its expression peaked on day 8 when the carpels first appeared. ‘PnCRC1’ showed greatest homology to Arabidopsis FILAMENTOUS (PnFIL). Its expression was approximately the same in inductive and non-inductive treatments. Apart from PnFIL these partial cDNAs could be used as markers to test the hypothesis concerning differential effects of sucrose and glucose. Cultured shoot apices from induced plants were sampled at weekly intervals. All four genes were expressed more strongly in the glucose compared with the sucrose treatment, most notably at day 17. A more intensive sampling (days 15–19) indicated that PnLFY and PnYABBY exhibited much higher expression on glucose compared with sucrose, most notably on days 15–16 and days 18–19.

Key words: Floral genes, flowering, glucose, Pharbitis nil, sucrose


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Plant Physiol.Home page
A. M. Price, D. F. Aros Orellana, F. M. Salleh, R. Stevens, R. Acock, V. Buchanan-Wollaston, A. D. Stead, and H. J. Rogers
A Comparison of Leaf and Petal Senescence in Wallflower Reveals Common and Distinct Patterns of Gene Expression and Physiology
Plant Physiology, August 1, 2008; 147(4): 1898 - 1912.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.