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JXB Advance Access originally published online on August 13, 2004
Journal of Experimental Botany 2004 55(406):2305-2312; doi:10.1093/jxb/erh236
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Journal of Experimental Botany, Vol. 55, No. 406, © Society for Experimental Biology 2004; all rights reserved

RESEARCH PAPER

Sulphur supply and infection with Pyrenopeziza brassicae influence L-cysteine desulphydrase activity in Brassica napus L.

Elke Bloem1,*, Anja Riemenschneider2, Julia Volker2, Jutta Papenbrock2,*, Ahlert Schmidt2, Ioana Salac1, Silvia Haneklaus1 and Ewald Schnug1

1Institute for Plant Nutrition and Soil Science, Federal Agricultural Research Centre (FAL), Bundesallee 50, D-38116 Braunschweig, Germany
2Institute for Botany, University of Hanover, Herrenhäuserstr. 2, D-30419 Hanover, Germany

* To whom correspondence should be addressed. Elke Bloem: Fax: +49 531 596 2199. E-mail: elke.bloem{at}fal.de; Jutta Papenbrock: Fax: + 49 511 762 3992. E-mail: Jutta.Papenbrock{at}botanik.uni-hannover.de

Different field surveys have shown that sulphur (S) fertilization can increase the resistance of agricultural crops against fungal pathogens. The mechanisms of this sulphur-induced resistance (SIR) are, however, not yet known. Volatile S compounds are thought to play an important role because H2S is toxic to fungi. A field experiment was conducted to analyse the influence of S fertilization and the activity of H2S-releasing enzymes on fungal infections. Two levels of N and S fertilizers and two varieties of oilseed rape were investigated with respect to their potential to release H2S by the enzymatic activity of L-cysteine desulphydrase (LCD) and O-acetyl-L-serine(thiol)lyase (OAS-TL). LCD releases H2S during cysteine degradation, while OAS-TL consumes H2S during cysteine synthesis and free H2S is only released in a side reaction. All plots of the field trial showed an infection with Pyrenopeziza brassicae and leaf disc samples were taken from visibly infected leaf areas and apparently uninfected areas to investigate the reaction to the infection in relation to the treatments. Different S fractions and the activities of LCD and OAS-TL were measured to evaluate the potential to release H2S in relation to S nutrition and fungal infection. S fertilization significantly increased the contents of total S, sulphate, organic S, cysteine, and glutathione in the plants, but decreased LCD activity. Infection with P. brassicae increased cysteine and glutathione contents, as well as the activity of LCD. Therefore crops were able to react to a fungal infection with a greater potential to release H2S, which is reflected by an increasing LCD activity with fungal infection.

Key words: Brassica napus L., H2S, L-cysteine desulphydrase, O-acetyl-L-serine(thiol)lyase, Pyrenopeziza brassicae, sulphur-induced resistance


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