Skip Navigation


JXB Advance Access originally published online on June 27, 2005
Journal of Experimental Botany 2005 56(418):2173-2181; doi:10.1093/jxb/eri217
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
56/418/2173    most recent
eri217v1
Right arrow E-letters: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when E-letters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (4)
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Amritphale, D.
Right arrow Articles by Kusumoto, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Amritphale, D.
Right arrow Articles by Kusumoto, D.
Agricola
Right arrow Articles by Amritphale, D.
Right arrow Articles by Kusumoto, D.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author [2005]. Published by Oxford University Press [on behalf of the Society for Experimental Biology]. All rights reserved. For Permissions, please e-mail: journals.permissions@oupjournals.org

RESEARCH PAPER

The modulating effect of the perisperm–endosperm envelope on ABA-inhibition of seed germination in cucumber

Dilip Amritphale1,*, Koichi Yoneyama2, Yasutomo Takeuchi2, P. Ramakrishna1 and Dai Kusumoto2

1Institute of Environment Management and Plant Sciences, Vikram University, Ujjain (MP) 456 010, India
2Center for Research on Wild Plants, Utsunomiya University, 350 Mine-machi, Utsunomiya 321-8505, Japan

* To whom correspondence should be addressed. Fax: +91 0734 2511226. E-mail: dilipamr{at}sancharnet.in; dilipamritphale{at}yahoo.co.in

Abscisic acid (ABA) markedly reduced the germination of developing seeds at much lower concentrations (ABA50=0.1 mM) compared with that of mature seeds (ABA50=1.6 mM) in cucumber (Cucumis sativus L. cv. Green long). The perisperm–endosperm (PE) envelope in developing seeds showed partly differentiated lipid and callose layers, considerable ABA biosynthetic activity in endosperm cells, and appreciable permeability to applied ABA. The decrease in the sensitivity of seeds to applied ABA was coincident with the complete development of lipid and callose layers, diminished ABA biosynthetic activity in endosperm cells in imbibed mature seeds, and moderate permeability of the PE envelope to applied ABA. Decoated seeds pretreated with chloroform showed decreased germination (ABA50=0.4 mM) in response to applied ABA and increased ABA permeation through the PE envelope. ABA thus allowed to permeate into embryonic tissues substantially reduced the pregerminative activity of ß-glucanase in the radicles. The structure and biophysical/biochemical properties of the PE envelope seem to modulate the effect of ABA on the germination of developing and mature cucumber seeds.

Key words: Abscisic acid, callose, chloroform treatment, cucumber, Cucumis sativus, perisperm–endosperm envelope, permeability, seed germination, wall hydrolases


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 ANN BOT (LOND)Home page
P. RAMAKRISHNA and D. AMRITPHALE
The Perisperm-endosperm Envelope in Cucumis: Structure, Proton Diffusion and Cell Wall Hydrolysing Activity
Ann. Bot., October 1, 2005; 96(5): 769 - 778.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.