JXB Advance Access originally published online on October 5, 2005
Journal of Experimental Botany 2005 56(421):3007-3016; doi:10.1093/jxb/eri298
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RESEARCH PAPER |
Characterization of transgenic Arabidopsis plants overexpressing GR-RBP4 under high salinity, dehydration, or cold stress
Division of Applied Plant Science and Agricultural Plant Stress Research Center, College of Agriculture and Life Sciences, Chonnam National University, 300 Youngbong-Dong, Buk-gu, Gwangju 500-757, South Korea
* To whom correspondence should be addressed. Fax: +82 62 530 2047. E-mail: hskang{at}chonnam.ac.kr
A glycine-rich RNA-binding protein4 (GR-RBP4), one of the eight GR-RBP family members in Arabidopsis thaliana, was investigated for its stress-related expression, nucleic acid-binding property, and functional roles in plants subjected to various stresses including cold, high salinity, and dehydration. Real-time RT-PCR and GUS expression analyses showed that GR-RBP4 was abundantly expressed in young plants, root tips, and flowers, but weakly in mature leaves and stems, implying that GR-RBP4 is highly expressed in actively proliferating organs. The transcript level of GR-RBP4 increased markedly with cold stress, decreased significantly with salt stress, and decreased slightly with dehydration stress. In vitro nucleic acid-binding assays revealed that GR-RBP4 protein binds sequence non-specifically to RNAs and DNAs. Characterization of the transgenic Arabidopsis plants overexpressing GR-RBP4 under the control of the 35S promoter revealed that 35S::GR-RBP4 lines displayed retarded germination compared with the wild type under salt or dehydration stress. Despite the marked up-regulation of GR-RBP4 expression by cold stress, the 35S::GR-RBP4 lines did not show any noticeable changes in cold or freezing tolerance compared with wild-type plants. These results indicate that GR-RBP4 contributes differently to altered germination and seedling growth of Arabidopsis plants under various stress conditions.
Key words: Glycine-rich RNA-binding protein, RNA-binding protein, stress, transgenic Arabidopsis thaliana plants
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