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JXB Advance Access originally published online on November 1, 2005
Journal of Experimental Botany 2005 56(422):3159-3169; doi:10.1093/jxb/eri313
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© The Author [2005]. Published by Oxford University Press [on behalf of the Society for Experimental Biology]. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

RESEARCH PAPER

A multifunctional bicupin serves as precursor for a chromosomal protein of Pisum sativum seeds

Josefa Castillo *, Ainhoa Genovés {dagger}, Luis Franco{ddagger} and M. Isabel Rodrigo

Department of Biochemistry and Molecular Biology, University of Valencia, E-46100, Burjassot, Valencia, Spain

{ddagger} To whom correspondence should be addressed. Fax: +34 96 354 4635. E-mail: luis.franco{at}uv.es

The fact that the psp54 gene codes for p16, a seed chromatin protein of Pisum sativum, has been described previously. In the present paper it is shown that p54, the p16 precursor, also exists as a free polypeptide in pea and that it also yields p38, a second polypeptide from the N-terminal region of p54, which is co-localized at a subcellular level with p16. By using antibodies against pea p16 and p38, it was found that these proteins are present in the members of the tribe Viciae examined. Sequence analysis and 3D modelling indicates that p54 proteins belong to the cupin superfamily, and that they are related to sucrose binding proteins and, to a lesser extent, to vicilin-type seed storage proteins. Nevertheless, several distinctive characteristics of psp54 expression have been found: (i) the gene is differentially induced by ABA and several stress situations, in accordance with the presence of putative separate ABA and stress responsive elements in its promoter; (ii) the proteins are present in pods and seed coats, tissues of maternal origin; and (iii) p54 mRNA accumulates in the dry seeds. In view of both the functional properties of p54-derived proteins and the features of the psp54 gene expression, it is concluded that p54 represents a novel class within the cupin superfamily.

Key words: Abscisic acid, ABA, cupins, pea seed germination, processing of precursor peptides, protein modelling, stress-induced gene expression


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