Skip Navigation


JXB Advance Access originally published online on July 25, 2006
Journal of Experimental Botany 2006 57(11):2847-2865; doi:10.1093/jxb/erl043
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrowOA All Versions of this Article:
57/11/2847    most recent
erl043v1
Right arrow E-letters: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when E-letters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (11)
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Moscatiello, R.
Right arrow Articles by Maathuis, F. J. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Moscatiello, R.
Right arrow Articles by Maathuis, F. J. M.
Agricola
Right arrow Articles by Moscatiello, R.
Right arrow Articles by Maathuis, F. J. M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© 2006 The Author(s).
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)

RESEARCH PAPER

Transcriptional analysis of calcium-dependent and calcium-independent signalling pathways induced by oligogalacturonides

Roberto Moscatiello1, Paola Mariani1, Dale Sanders2 and Frans J. M. Maathuis2,*

1Department of Biology, University of Padua, Via Ugo Bassi 58b, I-35131 Padova, Italy
2Department of Biology (Area 9), University of York, PO Box 373, York YO10 5YW, UK

*To whom correspondence should be addressed. E-mail: fjm3{at}york.ac.uk

{alpha}-1,4-linked oligogalacturonides (OGs) are pectic fragments of plant cell walls that are able to induce defence and developmental responses. To understand plant responses to OGs at the transcriptional level, changes in gene expression were examined using oligonucleotide-based microarrays that cover almost the entire Arabidopsis transcriptome. In suspension-cultured Arabidopsis thaliana (L.) Columbia hypocotyl cells, approximately 4% of the total transcriptome exhibited significant change in abundance in response to treatment with OGs for 2 h. Steady-state changes in the abundance of transcripts encoding stress- and disease-related proteins, signalling components, and transcription factors were particularly noteworthy. As in other plant cell types, OGs elicit a rapid, but transient, elevation in cytosolic free Ca2+. The Ca2+ transient can be abolished by the protein kinase inhibitor 4,5,6,7-tetrabromobenzotriazole (TBB) and by the Ca2+ channel inhibitor La3+, thereby facilitating a distinction between Ca2+-dependent and -independent transcriptional responses. Among the 244 transcripts that were up-regulated by OGs, the response of 93 (38%) was selectively sensitive to abolition of the Ca2+ transient. These OG-up-regulated, Ca2+-dependent transcripts included two noteworthy classes, the first comprising genes involved in cell wall modification following pathogen attack, and the second consisting of genes involved in the biosynthesis of jasmonate and C6 volatile compounds. These results support the notion of an important role for cytosolic Ca2+ signalling in jasmonate biosynthesis following OG perception. Promoter analysis of OG-induced, inhibitor-sensitive and -insensitive genes identified several putative cis-elements that might be involved specifically in Ca2+-dependent transcriptional regulation.

Key words: Arabidopsis, calcium, oligogalacturonides, signalling, transcriptomics


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J Exp BotHome page
P. Senadheera, R. K. Singh, and F. J. M. Maathuis
Differentially expressed membrane transporters in rice roots may contribute to cultivar dependent salt tolerance
J. Exp. Bot., July 1, 2009; 60(9): 2553 - 2563.
[Abstract] [Full Text] [PDF]

Home page
 Mol PlantHome page
C. Denoux, R. Galletti, N. Mammarella, S. Gopalan, D. Werck, G. De Lorenzo, S. Ferrari, F. M. Ausubel, and J. Dewdney
Activation of Defense Response Pathways by OGs and Flg22 Elicitors in Arabidopsis Seedlings
Mol Plant, May 22, 2008; (2008) ssn019v1.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
L. Navazio, R. Moscatiello, A. Genre, M. Novero, B. Baldan, P. Bonfante, and P. Mariani
A Diffusible Signal from Arbuscular Mycorrhizal Fungi Elicits a Transient Cytosolic Calcium Elevation in Host Plant Cells
Plant Physiology, June 1, 2007; 144(2): 673 - 681.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
A. Aziz, A. Gauthier, A. Bezier, B. Poinssot, J.-M. Joubert, A. Pugin, A. Heyraud, and F. Baillieul
Elicitor and resistance-inducing activities of {beta}-1,4 cellodextrins in grapevine, comparison with {beta}-1,3 glucans and {alpha}-1,4 oligogalacturonides
J. Exp. Bot., April 1, 2007; 58(6): 1463 - 1472.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
J. Wu, C. Hettenhausen, S. Meldau, and I. T. Baldwin
Herbivory Rapidly Activates MAPK Signaling in Attacked and Unattacked Leaf Regions but Not between Leaves of Nicotiana attenuata
PLANT CELL, March 1, 2007; 19(3): 1096 - 1122.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.