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JXB Advance Access originally published online on August 1, 2006
Journal of Experimental Botany 2006 57(12):3057-3067; doi:10.1093/jxb/erl067
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© The Author [2006]. Published by Oxford University Press [on behalf of the Society for Experimental Biology]. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

RESEARCH PAPER

Temperature response of photosynthesis and internal conductance to CO2: results from two independent approaches

CR Warren1,* {dagger} and E Dreyer2

1School of Forest and Ecosystem Science, University of Melbourne, Water Street, Creswick, VIC 3363, Australia
2INRA, UMR INRA-UHP, Ecologie et Ecophysiologie Forestières, F-54280 Champenoux, France

*To whom correspondence should be addressed. E-mail: charles.warren{at}bio.usyd.edu.au

The internal conductance to CO2 transfer from intercellular spaces to chloroplasts poses a major limitation to photosynthesis, but few studies have investigated its temperature response. The aim of this study was to determine the temperature response of photosynthesis and internal conductance between 10 °C and 35 °C in seedlings of a deciduous forest tree species, Quercus canariensis. Internal conductance was estimated via simultaneous measurements of gas exchange and chlorophyll fluorescence (‘variable J method’). Two of the required parameters, the intercellular photocompensation point (Ci*) and rate of mitochondrial respiration in the light (Rd), were estimated by the Laisk method. These were used to calculate the chloroplastic photocompensation point ({Gamma}*) in a simultaneous equation with gi. An independent estimate of internal conductance was obtained by a novel curve-fitting method based on the curvature of the initial Rubisco-limited portion of an A/Ci curve. The temperature responses of the rate of Rubisco carboxylation (Vcmax) and the RuBP limited rate of electron transport (Jmax) were determined from chloroplastic CO2 concentrations. The rate of net photosynthesis peaked at 24 °C. Ci* was similar to reports for other species with a Ci* of 39 µmol mol–1 at 25 °C and an activation energy of 34 kJ mol–1. {Gamma}* was very similar to the published temperature response for Spinacia oleracea from 20 °C to 35 °C, but was slightly greater at 10 °C and 15 °C. Jmax peaked at 30 °C, whereas Vcmax did not reach a maximum between 10 °C and 35 °C. Activation energies were 49 kJ mol–1 for Vcmax and 100 kJ mol–1 for Jmax. Both methods showed that internal conductance doubled from 10 °C to 20 °C, and then was nearly temperature-independent from 20 °C to 35 °C. Hence, the temperature response of internal conductance could not be fitted to an Arrhenius function. The best fit to estimated gi was obtained with a three-parameter log normal function (R2=0.98), with a maximum gi of 0.19 mol m–2 s–1 at 29 °C.

Key words: Carbon dioxide, diffusion, internal resistance, mesophyll resistance, photosynthesis, temperature responses, transfer conductance, transfer resistance


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