Skip Navigation


JXB Advance Access originally published online on September 12, 2006
Journal of Experimental Botany 2006 57(14):3583-3594; doi:10.1093/jxb/erl104
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow Supplementary Material
Right arrow All Versions of this Article:
57/14/3583    most recent
erl104v1
Right arrow E-letters: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when E-letters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (1)
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Yu, L.
Right arrow Articles by Moran, N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yu, L.
Right arrow Articles by Moran, N.
Agricola
Right arrow Articles by Yu, L.
Right arrow Articles by Moran, N.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author [2006]. Published by Oxford University Press [on behalf of the Society for Experimental Biology]. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

RESEARCH PAPER

Phosphorylation of SPICK2, an AKT2 channel homologue from Samanea motor cells

Ling Yu1 *, Dirk Becker2 *, Hadas Levi1, Menachem Moshelion1, Rainer Hedrich2, Ilana Lotan3, Arie Moran4, Uri Pick5, Leah Naveh1, Yael Libal1 and Nava Moran1,{dagger}

1The Robert H. Smith Institute for Plant Sciences and Genetics in Agriculture, Faculty of Agricultural, Food and Environmental Quality Sciences, the Hebrew University of Jerusalem, Rehovot 76100, Israel
2Julius-von-Sachs-Insitute, Department of Botany I: Molecular Plant Physiology and Biophysics, University of Wuerzburg, Julius-von-Sachs-Platz 2, D-97082 Wuerzburg, Germany
3Department of Physiology and Pharmacology, Sackler School of Medicine, Tel Aviv University, Tel Aviv, Israel
4Department of Physiology, Faculty of Health Sciences, Ben-Gurion University, Beer Sheva, Israel
5Department of Biological Chemistry, Weizmann Instititute of Science, Rehovot 76100, Israel

{dagger} To whom correspondence should be addressed. E-mail: nava.moran{at}huji.ac.il

SPICK2, a homologue of the weakly-inward-rectifying Shaker-like Arabidopsis K channel, AKT2, is a candidate K+-influx channel participating in light- and clock-regulated leaf movements of the legume, Samanea saman. Light and the biological clock regulate the in situ K+-influx channel activity differentially in extensor and flexor halves of the pulvinus (the S. saman leaf motor organ), and also—though differently—the transcript level of SPICK2 in the pulvinus. This disparity between the in situ channel activity versus its candidate transcript, along with the sequence analysis of SPICK2, suggest an in situ regulation of the activity of SPICK2, possibly by phosphorylation and/or by interaction with cAMP. Consistent with this (i) the activity of the voltage-dependent K+-selective fraction of the inward current in extensor and flexor cells was affected differentially in whole-cell patch–clamp assays promoting phosphorylation (using the protein phosphatase inhibitor okadaic acid); (ii) several proteins in isolated plasma membrane-enriched vesicles of the motor cells underwent phosphorylation without an added kinase in conditions similar to patch–clamp; and (iii) the SPICK2 protein was phosphorylated in vitro by the catalytic subunit of the broad-range cAMP-dependent protein kinase. All of these results are consistent with the notion that SPICK2 is the K+-influx channel, and is regulated in vivo directly by phosphorylation.

Key words: AKT2, gating, kinase, motor cells, nucleotides, phosphorylation, PKA, potassium channel, selectivity, Samanea


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.