Skip Navigation


JXB Advance Access originally published online on September 6, 2006
Journal of Experimental Botany 2006 57(14):3639-3645; doi:10.1093/jxb/erl103
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
57/14/3639    most recent
erl103v1
Right arrow E-letters: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when E-letters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (9)
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Gerjets, T.
Right arrow Articles by Sandmann, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gerjets, T.
Right arrow Articles by Sandmann, G.
Agricola
Right arrow Articles by Gerjets, T.
Right arrow Articles by Sandmann, G.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author [2006]. Published by Oxford University Press [on behalf of the Society for Experimental Biology]. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

RESEARCH PAPER

Ketocarotenoid formation in transgenic potato

Tanja Gerjets and Gerhard Sandmann*

Molecular Biosciences 213, J.W. Goethe Universität, PO Box 111932, D-60054 Frankfurt/M., Germany

* To whom correspondence should be addressed. E-mail: sandmann{at}em.uni-frankfurt.de

Potato has been genetically engineered for the production of commercially important ketocarotenoids including astaxanthin (3,3'-dihydroxy 4,4'-diketo-ß-carotene). To support the formation of 3-hydroxylated and 4-ketolated ß-carotene, a transgenic potato line accumulating zeaxanthin due to inactivated zeaxanthin epoxidase was co-transformed with the crtO ß-carotene ketolase gene from the cyanobacterium Synechocystis under a constitutive promoter. Plants were generated which exhibited expression of this gene, resulting in an accumulation of echinenone, 3'-hydroxyechinenone, and 4-ketozeaxanthin in leaves, as well as 3'-hydroxyechinenone, 4-ketozeaxanthin together with astaxanthin in the tuber. The amount of ketocarotenoids formed represent ~10–12% of total carotenoids in leaves and tubers. Negative effects on photosynthesis due to the presence of the ketocarotenoids in leaves could be excluded by the determination of variable fluorescence.

Key words: Astaxanthin, ketocarotenoids, ketolase gene crtO, transgenic potato, tuber carotenoids


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J Exp BotHome page
A. B. Lopez, J. Van Eck, B. J. Conlin, D. J. Paolillo, J. O'Neill, and L. Li
Effect of the cauliflower Or transgene on carotenoid accumulation and chromoplast formation in transgenic potato tubers
J. Exp. Bot., February 5, 2008; (2008) erm299v1.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.