Skip Navigation


JXB Advance Access originally published online on September 12, 2006
Journal of Experimental Botany 2006 57(14):3659-3667; doi:10.1093/jxb/erl113
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
57/14/3659    most recent
erl113v1
Right arrow E-letters: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when E-letters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (7)
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Galmés, J.
Right arrow Articles by Flexas, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Galmés, J.
Right arrow Articles by Flexas, J.
Agricola
Right arrow Articles by Galmés, J.
Right arrow Articles by Flexas, J.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author [2006]. Published by Oxford University Press [on behalf of the Society for Experimental Biology]. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

RESEARCH PAPER

Acclimation of Rubisco specificity factor to drought in tobacco: discrepancies between in vitro and in vivo estimations

Jeroni Galmés, Hipólito Medrano and Jaume Flexas*

Laboratori de Fisiologia Vegetal, Grup de Biologia de les Plantes en Condicions Mediterrànies, Universitat de les Illes Balears. Carretera de Valldemossa Km 7.5, E-07122 Palma de Mallorca, Balears, Spain

* To whom correspondence should be addressed. E-mail: jaume.flexas{at}uib.es

In studies about the photosynthesis response to environmental stresses, such as drought, the Rubisco specificity factor ({tau}) is assumed to be constant or derived indirectly from gas exchange measurements. However, an analysis of the acclimation of {tau} to drought using in vitro determinations is lacking. The aim of the present work was to analyse the acclimation of {tau} to different drought intensities in tobacco (Nicotiana tabacum L.). Potted tobacco plants were subjected to three different water regimes (100%, 40%, and 15% of field capacity) and new leaves were allowed to develop. When acclimated leaves were fully developed, they were sampled for gas exchange and chlorophyll fluorescence measurements, as well as for the in vitro analysis of Rubisco kinetic properties. Relative water content and gas exchange decreased with increasing water shortage. The apparent Rubisco specificity factor as estimated in vivo by gas exchange decreased with water stress. However, in vitro estimates of {tau} were identical among treatments, as were Rubisco specific initial activity and activation state. The reasons for the observed discrepancy between in vitro and in vivo estimates are profusely discussed. It is suggested that the Rubisco specificity factor does not acclimate to water stress in the short term (weeks or months) in tobacco, and the validity of the so-called Laisk gas exchange method to estimate {tau} under drought is questioned.

Key words: Acclimation, drought, Nicotiana tabacum, Rubisco, specificity factor, water stress


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J Exp BotHome page
J. Flexas, M. Baron, J. Bota, J.-M. Ducruet, A. Galle, J. Galmes, M. Jimenez, A. Pou, M. Ribas-Carbo, C. Sajnani, et al.
Photosynthesis limitations during water stress acclimation and recovery in the drought-adapted Vitis hybrid Richter-110 (V. berlandierixV. rupestris)
J. Exp. Bot., May 1, 2009; 60(8): 2361 - 2377.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
U. Niinemets, A. Diaz-Espejo, J. Flexas, J. Galmes, and C. R. Warren
Role of mesophyll diffusion conductance in constraining potential photosynthetic productivity in the field
J. Exp. Bot., May 1, 2009; 60(8): 2249 - 2270.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
A. Perez-Martin, J. Flexas, M. Ribas-Carbo, J. Bota, M. Tomas, J. M. Infante, and A. Diaz-Espejo
Interactive effects of soil water deficit and air vapour pressure deficit on mesophyll conductance to CO2 in Vitis vinifera and Olea europaea
J. Exp. Bot., May 1, 2009; 60(8): 2391 - 2405.
[Abstract] [Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
O. K. Atkin and D. Macherel
The crucial role of plant mitochondria in orchestrating drought tolerance
Ann. Bot., February 1, 2009; 103(4): 581 - 597.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
C. R. Warren
Soil water deficits decrease the internal conductance to CO2 transfer but atmospheric water deficits do not
J. Exp. Bot., February 1, 2008; 59(2): 327 - 334.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
J Flexas, A Diaz-Espejo, J. Berry, J Cifre, J Galmes, R Kaldenhoff, H Medrano, and M Ribas-Carbo
Analysis of leakage in IRGA's leaf chambers of open gas exchange systems: quantification and its effects in photosynthesis parameterization
J. Exp. Bot., April 1, 2007; 58(6): 1533 - 1543.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.