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JXB Advance Access originally published online on October 17, 2006
Journal of Experimental Botany 2006 57(14):3781-3791; doi:10.1093/jxb/erl191
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© The Author [2006]. Published by Oxford University Press [on behalf of the Society for Experimental Biology]. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

RESEARCH PAPER

Respiratory Burst Oxidase Homologue A of barley contributes to penetration by the powdery mildew fungus Blumeria graminis f. sp. hordei

Marco Trujillo1 *, Lothar Altschmied2, Patrick Schweizer2, Karl-Heinz Kogel1 and Ralph Hückelhoven1,{dagger}

1Research Centre for BioSystems, Land Use and Nutrition, Institute of Phytopathology and Applied Zoology, Justus-Liebig-University of Giessen, Heinrich-Buff Ring 26–32, D-35392 Giessen, Germany
2The Institute of Plant Genetics and Crop Plant Research, D-06466 Gatersleben, Germany

{dagger} To whom correspondence should be addressed. E-mail: ralph.hueckelhoven{at}agrar.uni-giessen.de

Reactive oxygen intermediates (ROI) are closely related to defence reactions of plants against pathogens. A prominent role in the production of ROI has been attributed to the plant respiratory burst oxidase homologues (RBOH) of the human phagocyte GP91(phox). A barley RBOH, which encodes a putative superoxide (O2·–) producing NADPH oxidase, is described here. Histochemical analysis of the barley-Blumeria graminis f. sp. hordei (Bgh) interaction showed that O2·– is produced locally at the site of penetration. In contrast, hydrogen peroxide (H2O2) is produced in non-penetrated cell wall appositions. A barley RBOHA cDNA was isolated and a minor induction of expression of RBOHA was observed during the interactions of barley with Bgh. Transient RNA interference-mediated gene silencing of HvRBOHA during the penetration process of Bgh led to an increase of basal penetration resistance. The results support a potential role of HvRBOHA in cellular accessibility to Blumeria graminis.

Key words: Blumeria graminis, cell wall softening, hydrogen peroxide, HvRBOH, penetration, resistance, superoxide (O2·–), susceptibility


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