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JXB Advance Access originally published online on January 5, 2006
Journal of Experimental Botany 2006 57(3):537-545; doi:10.1093/jxb/erj036
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© The Author [2006]. Published by Oxford University Press [on behalf of the Society for Experimental Biology]. All rights reserved. The online version of this article has been published under an Open Access model. Users are entitled to use, reproduce, disseminate, or display the Open Access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and the Society for Experimental Biology are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact: journals.permissions@oxfordjournals.org

RESEARCH PAPER

Increased extensin levels in Arabidopsis affect inflorescence stem thickening and height

K. Roberts1 and A. H. Shirsat2,*

1The Scottish Crop Research Institute, University of Dundee, Invergowrie, Dundee DD2 5DA, UK
2School of Biological Sciences, Deiniol Road, University of Wales, Bangor, Gwynedd LL57 2UW, UK

* To whom correspondence should be addressed. E-mail: a.h.shirsat{at}bangor.ac.uk

Extensins are hydroxyproline-rich glycoproteins which are abundant in the cell walls of higher plants and whose precise function has been the subject of much speculation. In order to investigate this, transgenic A. thaliana plants were generated containing the Arabidopsis Atext1 extensin coding sequence under the transcriptional control of the strong constitutive CaMV 35S promoter. Northern analysis and RT-PCR identified transgenics with high constitutive levels of Atext1 mRNA. Hydroxyproline assays confirmed that transgenic plants which over-expressed Atext1 contained high levels of hydroxyproline-rich protein. Phenotypic analysis of the transgenics showed that there were no significant phenotypic effects on the timing of different developmental stages or on the general form of the plant. However, transgenics with high extensin levels showed an enhanced increase in stem thickness, with an indirect effect on stem height. This effect, is, however, rather small, with a reduction in height of between 5–8% depending on the transgenic line being analysed.

Key words: Arabidopsis, Atext1, cell wall, extensin, hydroxyproline, phenotype, stem thickness


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