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JXB Advance Access originally published online on January 12, 2006
Journal of Experimental Botany 2006 57(3):581-588; doi:10.1093/jxb/erj045
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© The Author [2006]. Published by Oxford University Press [on behalf of the Society for Experimental Biology]. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

RESEARCH PAPER

Nitric oxide modulates the expression of cell cycle regulatory genes during lateral root formation in tomato

Natalia Correa-Aragunde1, Magdalena Graziano1, Christian Chevalier2 and Lorenzo Lamattina1,*

1Instituto de Investigaciones Biológicas, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata, CC 1245, 7600 Mar del Plata, Argentina
2Unité Mixte de Recherche 0619 en Physiologie et Biotechnologie Végétales (Institut National de la Recherche Agronomique et Universités de Bordeaux 1 et Victor Segalen-Bordeaux 2) Centre de Recherche INRA de Bordeaux, BP81, 33883 Villenave d'Ornon, cedex, France

* To whom correspondence should be addressed. E-mail: lolama{at}mdp.edu.ar

Nitric oxide (NO) is a bioactive molecule involved in diverse physiological functions in plants. It has previously been reported that the NO donor sodium nitroprusside (SNP) applied to germinated tomato seeds was able to induce lateral root (LR) formation in the same way that auxin treatment does. In this paper, it is shown that NO modulates the expression of cell cycle regulatory genes in tomato pericycle cells and leads, in turn, to induced LR formation. The addition of the NO scavenger CPTIO at different time points during auxin-mediated LR development indicates that NO is required for LR primordia formation and not for LR emergence. The SNP-mediated LR promotion could be prevented by the cell cycle inhibitor olomoucine, suggesting that NO is involved in cell cycle regulation. A system was developed in which the formation of LRs was synchronized. It was based on the control of NO availability in roots by treatment with the NO scavenger CPTIO. The expression of the cell cycle regulatory genes encoding CYCA2;1, CYCA3;1, CYCD3;1, CDKA1, and the Kip-Related Protein KRP2 was studied using RT-PCR analysis in roots with synchronized and non-synchronized LR formation. NO mediates the induction of the CYCD3;1 gene and the repression of the CDK inhibitor KRP2 gene at the beginning of LR primordia formation. In addition, auxin-dependent cell cycle gene regulation was dependent on NO.

Key words: Auxin, cell cycle, cyclin, Cyclin Dependent Kinase, lateral root, nitric oxide


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