Skip Navigation


JXB Advance Access originally published online on March 17, 2006
Journal of Experimental Botany 2006 57(7):1553-1562; doi:10.1093/jxb/erj149
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
57/7/1553    most recent
erj149v1
Right arrow E-letters: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when E-letters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (15)
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Chivasa, S.
Right arrow Articles by Slabas, A. R
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chivasa, S.
Right arrow Articles by Slabas, A. R
Agricola
Right arrow Articles by Chivasa, S.
Right arrow Articles by Slabas, A. R
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author [2006]. Published by Oxford University Press [on behalf of the Society for Experimental Biology]. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

RESEARCH PAPER

Proteomic analysis of differentially expressed proteins in fungal elicitor-treated Arabidopsis cell cultures

Stephen Chivasa, John M Hamilton, Richard S Pringle, Bongani K Ndimba, William J Simon, Keith Lindsey and Antoni R Slabas*

School of Biological and Biomedical Sciences, Durham University, Durham DH1 3LE, UK

*To whom correspondence should be addressed. E-mail: a.r.slabas{at}durham.ac.uk

Slow progress has been made in discovering plant genes governing the interaction of plant pathogens and their hosts using classical genetic approaches. Extensive studies employing DNA microarray techniques to identify global changes in gene expression during pathogen–host interaction have greatly enhanced discovery of genetic components regulating the plant defence response to pathogen attack. In this study, a complementary approach was used to identify changes in protein abundance during interaction of Arabidopsis cell cultures with a pathogen-derived elicitor. The soluble protein fractions were analysed by two-dimensional difference gel electrophoresis and proteins differentially expressed in response to treatment with fungal elicitor were identified via matrix-assisted laser desorption ionization–time of flight mass spectrometry. Elicitor responsive proteins included molecular chaperones, oxidative stress defence proteins, mitochondrial proteins, and enzymes of a diverse number of metabolic pathways. The findings, in combination with currently available microarray data, will form the basis of a filter to identify pivotal genes whose role in pathogen defence systems will require confirmation using gene knockout mutants.

Key words: Arabidopsis, 2-D DIGE, defence response, fungal elicitor, molecular chaperones, oxidative stress, plant pathogen, proteomic


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Cell Sci.Home page
C. A. Maxwell, J. McCarthy, and E. Turley
Cell-surface and mitotic-spindle RHAMM: moonlighting or dual oncogenic functions?
J. Cell Sci., April 1, 2008; 121(7): 925 - 932.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
Z. Deng, X. Zhang, W. Tang, J. A. Oses-Prieto, N. Suzuki, J. M. Gendron, H. Chen, S. Guan, R. J. Chalkley, T. K. Peterman, et al.
A Proteomics Study of Brassinosteroid Response in Arabidopsis
Mol. Cell. Proteomics, December 1, 2007; 6(12): 2058 - 2071.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
J. J. Benschop, S. Mohammed, M. O'Flaherty, A. J. R. Heck, M. Slijper, and F. L. H. Menke
Quantitative Phosphoproteomics of Early Elicitor Signaling in Arabidopsis
Mol. Cell. Proteomics, July 1, 2007; 6(7): 1198 - 1214.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.