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JXB Advance Access originally published online on May 26, 2007
Journal of Experimental Botany 2007 58(10):2503-2512; doi:10.1093/jxb/erm082
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© The Author [2007]. Published by Oxford University Press [on behalf of the Society for Experimental Biology]. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

RESEARCH PAPER

A sub-proteome of Arabidopsis thaliana mature stems trapped on Concanavalin A is enriched in cell wall glycoside hydrolases

Zoran Minic1, Elisabeth Jamet2, Luc Négroni3, P Arsene der Garabedian4, Michel Zivy3 and Lise Jouanin1,*

1Laboratoire de Biologie Cellulaire, Institut National de la Recherche Agronomique, Route de Saint-Cyr, F-78026 Versailles Cedex, France
2Surfaces Cellulaires et Signalisation chez les Végétaux, UMR 5546 CNRS-Université Paul Sabatier-Toulouse III, 24 Chemin de Borde Rouge, BP42617, F-31326-Castanet-Tolosan, France
3Institut National de la Recherche Agronomique, Centre National de la Recherche Scientifique, Université Paris XI, Institut National Agronomique Paris-Grignon, la Ferme du Moulon, F-91190 Gif-sur-Yvette, France
4Laboratoire d'Enzymologie des Acides Nucléiques, Université Pierre et Marie Curie, 96 Boulevard Raspail, F-75006 Paris, France

* To whom correspondence should be addressed. E-mail: jouanin{at}versailles.inra.fr

N-glycosylated proteins were isolated from Arabidopsis thaliana mature stems using affinity chromatography on Concanavalin A Sepharose, separated by 2D-electrophoresis and identified using nanoHPLC-MS/MS and MALDI-TOF MS. 102 glycoproteins were identified. 94% of these proteins were predicted by bioinformatics to be targeted to the secretory pathway and 87% of them were predicted to be localized in the cell wall or at the plasma membrane. 30% of these proteins belong to glycoside hydrolase (GH) families with some of them possibly involved in the hydrolysis of cell wall polysaccharides. The second major class of identified proteins comprises aspartyl and serine proteases. Other proteins are predicted to be oxido-reductases, contain interacting domains, are potentially involved in signalling or have an unknown function. This is, to our knowledge, the first survey of plant cell wall N-glycosylated proteins.

Key words: Arabidopsis thaliana, cell wall, Concanavalin A, glycoside hydrolase, glycosylation, plant stem

Received 24 January 2007; Revised 21 March 2007 Accepted 21 March 2007


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