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JXB Advance Access originally published online on December 5, 2006
Journal of Experimental Botany 2007 58(3):497-506; doi:10.1093/jxb/erl221
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© The Author [2006]. Published by Oxford University Press [on behalf of the Society for Experimental Biology]. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

RESEARCH PAPER

Overexpression of an auxilin-like gene (F9E10.5) can suppress Al uptake in roots of Arabidopsis

Bunichi Ezaki*, Hiroyuki Kiyohara, Hideaki Matsumoto and Susumu Nakashima

Research Institute for Bioresources, Okayama University, 2020-1, Chuou, Kurashiki, Okayama, 710-0046 Japan

* To whom correspondence should be addressed. E-mail: bezaki{at}rib.okayama-u.ac.jp

Plants resistant to aluminium (Al) stress were isolated from Arabidopsis thaliana enhancer-tagged mutant lines. Compared with the parental Col-7 control line, one of the resistant candidates, #355-2, showed a higher expression of the F9E10.5 gene (At1g75100) on chromosome 1, a lower Al content in whole roots, and a shorter root hair length (~30%). Both Al influx and associated oxidative stress occurred in root hairs, as well as in root tips of Col-7; however, they were seen only in root tips of #355-2. Transgenic plants overexpressing the F9E10.5 gene showed a slightly higher Al resistance than their parental control line (Ler). The F9E10.5 gene encodes an auxilin-like protein related to the clathrin-uncoating process in endocytosis. Microscopic observation indicated that both Al ion influx and endocytosis activity were lower in root hair cells of the #355-2 line than in those of Col-7. These results suggested that overexpression of this auxilin-like protein inhibits endocytosis in root hair cells by a disturbance of the transport system as in animal cells shown previously. It was also suggested that a part of the Al influx occurred via endocytosis in root hair cells in Arabidopsis. The Al resistance in the #355-2 line may therefore be due to a lower Al uptake via endocytosis in the root hair region.

Key words: Activation-tagged mutant line, aluminium (Al) resistance mechanism, Al influx, Arabidopsis thaliana, auxilin-like protein, endocytosis, oxidative stress, root hair

Received 30 August 2006; Revised 27 September 2006 Accepted 2 October 2006


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[Abstract] [Full Text] [PDF]


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