Skip Navigation


JXB Advance Access originally published online on September 27, 2006
Journal of Experimental Botany 2007 58(4):757-763; doi:10.1093/jxb/erl139
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow Supplementary Material
Right arrow All Versions of this Article:
58/4/757    most recent
erl139v2
erl139v1
Right arrow E-letters: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when E-letters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (3)
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Burrell, M.
Right arrow Articles by Clench, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Burrell, M.
Right arrow Articles by Clench, M.
Agricola
Right arrow Articles by Burrell, M.
Right arrow Articles by Clench, M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author [2006]. Published by Oxford University Press [on behalf of the Society for Experimental Biology]. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

Approaches to Cellular Imaging

Imaging Matrix Assisted Laser Desorption Ionization Mass Spectrometry: a technique to map plant metabolites within tissues at high spatial resolution

MM Burrell1,*, CJ Earnshaw2 and MR Clench2

1Department of Animal and Plant Sciences, Sheffield University, Western Bank, Sheffield S10 2TN, UK
2Biomedical Research Centre, Sheffield Hallam University, Howard Street, Sheffield S1 1WB, UK

* To whom correspondence should be addressed. E-mail: m.burrell{at}sheffield.ac.uk

Imaging Matrix Assisted Laser Desorption Ionization Mass Spectrometry provides a new and powerful tool to analyse the distribution of metabolites within plant tissues. The two matrices {alpha}-cyano-4-hydroxycinnamic acid ({alpha}-CHCA) and 9-aminoacridine provide a useful combination that allows the measurement of amino acids, sugars, and phosphorylated metabolites. Results are presented showing that representatives of these metabolites are unevenly distributed in wheat seeds at different stages of development and under temperature stress.

Key words: 9-aminoacridine, CHCA, I-MALDI, mass spectrometry, metabolites, phosphorylated metabolites, plants, sugars, wheat

Received 17 May 2006; Accepted 2 August 2006


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.