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JXB Advance Access originally published online on April 18, 2007
Journal of Experimental Botany 2007 58(7):1873-1892; doi:10.1093/jxb/erm012
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© The Author [2007]. Published by Oxford University Press [on behalf of the Society for Experimental Biology]. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

RESEARCH PAPER

Proteomic analysis reveals differences between Vitis vinifera L. cv. Chardonnay and cv. Cabernet Sauvignon and their responses to water deficit and salinity

Delphine Vincent1, Ali Ergül2, Marlene C. Bohlman1, Elizabeth A. R. Tattersall1, Richard L. Tillett1, Matthew D. Wheatley1, Rebekah Woolsey1, David R. Quilici1, Johann Joets3, Karen Schlauch4, David A. Schooley1, John C. Cushman1 and Grant R. Cramer1,*

1Department of Biochemistry and Molecular Biology, MS 200, University of Nevada, Reno, NV 89557, USA
2Institute of Biotechnology, University of Ankara, 06500 Besevler-Ankara, Turkey
3UMR de Genetique Vegetale, INRA/CNRS, F-91190 Gif-sur-Yvette, France
4Department of Genetics and Genomics, Boston University School of Medicine, Boston, MA 02118, USA

* To whom correspondence should be addressed. E-mail: cramer{at}unr.edu

The impact of water deficit and salt stress on two important wine grape cultivars, Chardonnay and Cabernet Sauvignon, was investigated. Plants were exposed to increasing salinity and water deficit stress over a 16 d time period. Measurements of stem water potentials, and shoot and leaf lengths indicated that Chardonnay was more tolerant to these stresses than Cabernet Sauvignon. Shoot tips were harvested every 8 d for proteomic analysis using a trichloroacetic acid/acetone extraction protocol and two-dimensional gel electrophoresis. Proteins were stained with Coomassie Brilliant Blue, quantified, and then 191 unique proteins were identified using matrix-assisted laser desorption ionization time of flight/time of flight mass spectrometry. Peptide sequences were matched against both the NCBI nr and TIGR Vitis expressed sequence tag (EST) databases that had been implemented with all public Vitis sequences. Approximately 44% of the protein isoforms could be identified. Analysis of variance indicated that varietal difference was the main source of protein expression variation (40%). In stressed plants, reduction of the amount of proteins involved with photosynthesis, protein synthesis, and protein destination was correlated with the inhibition of shoot elongation. Many of the proteins up-regulated in Chardonnay were of unclassified or of unknown function, whereas proteins specifically up-regulated in Cabernet Sauvignon were involved in protein metabolism.

Key words: 2-DE, grapevine, MALDI-TOF/TOF, salinity, shoot, water deficit

Received 29 August 2006; Revised 12 December 2006 Accepted 9 January 2007


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