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JXB Advance Access originally published online on May 29, 2008
Journal of Experimental Botany 2008 59(10):2781-2791; doi:10.1093/jxb/ern136
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© 2008 The Author(s).
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)

RESEARCH PAPER

Transcriptome profiling of ripening nectarine (Prunus persica L. Batsch) fruit treated with 1-MCP

Fiorenza Ziliotto1, Maura Begheldo1, Angela Rasori1, Claudio Bonghi1,* and Pietro Tonutti2,*

1Department of Environmental Agronomy and Crop Science, University of Padova, Viale dell’ Università 16, I-35025 Legnaro (Padova), Italy
2Sant'Anna School of Advanced Studies, Piazza Martiri della Libertà 33, I-56127 Pisa, Italy

* To whom correspondence should be addressed. E-mail: claudio.bonghi{at}unipd.it, pietro.tonutti{at}sssup.it

A large-scale transcriptome analysis has been conducted using µPEACH1.0 microarray on nectarine (Prunus persica L. Batsch) fruit treated with 1-methylcyclopropene (1-MCP). 1-MCP maintained flesh firmness but did not block ethylene biosynthesis. Compared with samples at harvest, only nine genes appeared to be differentially expressed when fruit were sampled immediately after treatment, while a total of 90 targets were up- or down-regulated in untreated fruit. The effect of 1-MCP was confirmed by a direct comparison of transcript profiles in treated and untreated fruit after 24 h of incubation with 106 targets differentially expressed. About 30% of these targets correspond to genes involved in primary metabolism and response processes related to ethylene, auxin, and other hormones. In treated fruit, altered transcript accumulation was detected for some genes with a role in ripening-related events such as softening, colour development, and sugar metabolism. A rapid decrease in flesh firmness and an increase in ethylene production were observed in treated fruit maintained for 48 h in air at 20 °C after the end of the incubation period. Microarray comparison of this sample with untreated fruit 24 h after harvest revealed that about 45% of the genes affected by 1-MCP at the end of the incubation period changed their expression during the following 48 h in air. Among these genes, an ethylene receptor (ETR2) and three ethylene-responsive factors (ERF) were present, together with other transcription factors and ethylene-dependent genes involved in quality parameter changes.

Key words: ACC synthase, ethylene biosynthesis, ethylene receptors, gene expression, microarray, 1-methylcyclopropene, post-harvest

Received 5 March 2008; Revised 18 April 2008 Accepted 21 April 2008


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