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JXB Advance Access originally published online on September 26, 2008
Journal of Experimental Botany 2008 59(13):3721-3728; doi:10.1093/jxb/ern219
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© 2008 The Author(s).
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)

RESEARCH PAPER

Response of plasma membrane H+-ATPase to heavy metal stress in Cucumis sativus roots

Malgorzata Janicka-Russak*, Katarzyna Kabala, Marek Burzynski and Grazyna Klobus

Department of Plant Physiology, Institute of Plant Biology, University of Wroclaw, Kanonia 6/8, 50–328 Wroclaw, Poland

* To whom correspondence should be addressed: E-mail: russak{at}biol.uni.wroc.pl

The effect of heavy metals on the modification of plasma membrane H+-ATPase (EC 3.6.3.14 [EC] ) activity in cucumber roots was studied. In plants stressed for 2 h with 10 µM or 100 µM Cd, Cu or Ni the hydrolytic as well as the transporting activity of H+-ATPase in the plasma membranes of root cells was decreased. Transcript levels of Cucumis sativus plasma membrane H+-ATPase in roots treated with 10 µM Cd, Cu, or Ni as well as with 100 µM Cu or Ni were similar to the control, indicating that the action of metals did not involve the gene expression level. Only in roots exposed to 100 µM Cd was the level of plasma membrane H+-ATPase mRNA markedly decreased. The inhibition of the plasma membrane proton pump caused by 100 µM Cd, Cu and Ni was partially diminished in the presence of cantharidin, a specific inhibitor of protein phosphatases. Western blot analysis with the antibody against phosphothreonine confirmed that decreased activity of plasma membrane H+-ATPase under heavy metals resulted from dephosphorylation of the enzyme protein. Taken together, these data strongly indicated that alteration of the enzyme under heavy metal stresses was mainly due to the post-translational modification of its proteins in short-term experiments.

Key words: Cd, Cu, H+-ATPase activity, heavy metals, Ni, plasma membrane

Received 2 April 2008; Revised 28 July 2008 Accepted 4 August 2008


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