Skip Navigation


JXB Advance Access originally published online on September 19, 2008
Journal of Experimental Botany 2008 59(14):3925-3939; doi:10.1093/jxb/ern234
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow Supplementary Material
Right arrowOA All Versions of this Article:
59/14/3925    most recent
ern234v1
Right arrow E-letters: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when E-letters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (2)
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Bomal, C.
Right arrow Articles by MacKay, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bomal, C.
Right arrow Articles by MacKay, J.
Agricola
Right arrow Articles by Bomal, C.
Right arrow Articles by MacKay, J.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© 2008 The Author(s).
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)

RESEARCH PAPER

Involvement of Pinus taeda MYB1 and MYB8 in phenylpropanoid metabolism and secondary cell wall biogenesis: a comparative in planta analysis

Claude Bomal1,*, Frank Bedon1,2, Sébastien Caron1, Shawn D. Mansfield4, Caroline Levasseur3, Janice E. K. Cooke6, Sylvie Blais1, Laurence Tremblay5, Marie-Josée Morency3, Nathalie Pavy1, Jacqueline Grima-Pettenati2, Armand Séguin3 and John MacKay1

1Centre d’Étude de la Forêt, Université Laval, Québec (QC), Canada G1V 0A6
2UMR UPS, CNRS 5546, Pôle de Biotechnologies Végétales, 24 chemin de Borde Rouge, BP42617, Auzeville Tolosane, 31326 Castanet Tolosan, France
3Natural Resources Canada, Canadian Forest Service, Laurentian Forestry Centre, Québec (QC), Canada G1V 4C7
4Canada Research Chair in Wood and Fibre Quality, Department of Wood Science, University of British Columbia, 4030-2424 Main Mall, Vancouver (BC), Canada V6T 1Z4
5DPSP, Ministère des Ressources naturelles et de la Faune, 2700, rue Einstein, Québec (QC), Canada G1P 3W8
6Department of Biological Sciences, CW405 Biological Sciences Building, University of Alberta, Edmonton (AB), Canada T6G 2E9

* To whom correspondence should be addressed. E-mail: bomalc{at}rsvs.ulaval.ca

The involvement of two R2R3-MYB genes from Pinus taeda L., PtMYB1 and PtMYB8, in phenylpropanoid metabolism and secondary cell wall biogenesis was investigated in planta. These pine MYBs were constitutively overexpressed (OE) in Picea glauca (Moench) Voss, used as a heterologous conifer expression system. Morphological, histological, chemical (lignin and soluble phenols), and transcriptional analyses, i.e. microarray and reverse transcription quantitative PCR (RT-qPCR) were used for extensive phenotyping of MYB-overexpressing spruce plantlets. Upon germination of somatic embryos, root growth was reduced in both transgenics. Enhanced lignin deposition was also a common feature but ectopic secondary cell wall deposition was more strongly associated with PtMYB8-OE. Microarray and RT-qPCR data showed that overexpression of each MYB led to an overlapping up-regulation of many genes encoding phenylpropanoid enzymes involved in lignin monomer synthesis, while misregulation of several cell wall-related genes and other MYB transcription factors was specifically associated with PtMYB8-OE. Together, the results suggest that MYB1 and MYB8 may be part of a conserved transcriptional network involved in secondary cell wall deposition in conifers.

Received 2 June 2008; Revised 15 August 2008 Accepted 19 August 2008


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.