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JXB Advance Access originally published online on November 13, 2008
Journal of Experimental Botany 2008 59(15):4145-4159; doi:10.1093/jxb/ern256
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© 2008 The Author(s).
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)

RESEARCH PAPER

Molecular analysis of post-harvest withering in grape by AFLP transcriptional profiling

Anita Zamboni1, Leone Minoia2, Alberto Ferrarini2, Giovanni Battista Tornielli1, Elisa Zago2, Massimo Delledonne2 and Mario Pezzotti1,*

1Department for Sciences, Technologies and Markets of Grapevine and Wine, Via della Pieve 70, I-37029 San Floriano di Valpolicella (VR), Italy
2Scientific and Technologic Department, University of Verona, Strada Le Grazie 15, I-37134 Verona, Italy

* To whom correspondence should be addressed: E-mail: mario.pezzotti{at}univr.it

Post-harvest withering of grape berries is used in the production of dessert and fortified wines to alter must quality characteristics and increase the concentration of simple sugars. The molecular processes that occur during withering are poorly understood, so a detailed transcriptomic analysis of post-harvest grape berries was carried out by AFLP-transcriptional profiling analysis. This will help to elucidate the molecular mechanisms of berry withering and will provide an opportunity to select markers that can be used to follow the drying process and evaluate different drying techniques. AFLP-TP identified 699 withering-specific genes, 167 and 86 of which were unique to off-plant and on-plant withering, respectively. Although similar molecular events were revealed in both withering processes, it was apparent that off-plant withering induced a stronger dehydration stress response resulting in the high level expression of genes involved in stress protection mechanisms, such as dehydrin and osmolite accumulation. Genes involved in hexose metabolism and transport, cell wall composition, and secondary metabolism (particularly the phenolic and terpene compound pathways) were similarly regulated in both processes. This work provides the first comprehensive analysis of the molecular events underpinning post-harvest withering and could help to define markers for different withering processes.

Key words: AFLP-TP, gene expression, grape berry withering, on- and off-plant withering processes

Received 23 June 2008; Revised 17 September 2008 Accepted 18 September 2008


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