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JXB Advance Access originally published online on January 22, 2008
Journal of Experimental Botany 2008 59(2):121-133; doi:10.1093/jxb/erm289
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© The Author [2008]. Published by Oxford University Press [on behalf of the Society for Experimental Biology]. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

FOCUS PAPER

Impact of chloroplastic- and extracellular-sourced ROS on high light-responsive gene expression in Arabidopsis

Ulrike Bechtold1, Odile Richard2, Alessandro Zamboni1 *, Catherine Gapper3, Matt Geisler4 {dagger}, Barry Pogson5, Stanislaw Karpinski4 and Philip M. Mullineaux1,{ddagger}

1Department of Biological Sciences, University of Essex, Colchester CO4 3SQ, UK
2Laboratoire de Biochimie et Physiologie Moléculaire des Plantes, Institut de Biologie Intégrative des Plantes, CNRS, Place Viala, 34060 Montpellier cedex 1, France
3John Innes Centre, Colney, Norwich NR4 7UH, UK
4Department of Botany, Stockholm University, 106 91 Stockholm, Sweden
5ARC Centre of Excellence in Plant Energy Biology, School of Biochemistry and Molecular Biology, The Australian National University, Canberra, ACT 0200, Australia

{ddagger} To whom correspondence should be addressed. E-mail: mullin{at}essex.ac.uk

The expression of 28 high light (HL)-responsive genes of Arabidopsis was analysed in response to environmental and physiological factors known to influence the expression of the HL-responsive gene, ASCORBATE PEROXIDASE2 (APX2). Most (81%) of the HL-responsive genes, including APX2, required photosynthetic electron transport for their expression, and were responsive to abscisic acid (ABA; 68%), strengthening the impression that these two signals are crucial in the expression of HL-responsive genes. Further, from the use of mutants altered in reactive oxygen species (ROS) metabolism, it was shown that 61% of these genes, including APX2, may be responsive to chloroplast-sourced ROS. In contrast, apoplastic/plasma membrane-sourced H2O2, in part directed by the respiratory burst NADPH oxidases AtrbohD and AtrbohF, was shown to be important only for APX2 expression. APX2 expression in leaves is limited to bundle sheath parenchyma; however, for the other genes in this study, information on their tissue specificity of expression is sparse. An analysis of expression in petioles, enriched for bundle sheath tissue compared with distal leaf blade, in HL and control leaves showed that 25% of them had >10-fold higher expression in the petiole than in the leaf blade. However, this did not mean that these petiole expression genes followed a pattern of regulation observed for APX2.

Key words: Arabidopsis, chloroplast, excess light, gene expression, plasma membrane, reactive oxygen species, signalling

* Present address: Dipartimento di Colture Arboree, Università di Bologna, Viale G. Fanin 46, 40127 Bologna, Italy.

{dagger} Present address: Department of Biochemistry, Schulich School of Medicine and Dentistry, University of Western Ontario. London, Ontario, Canada N6A 5C1.

Received 14 June 2007; Revised 2 October 2007 Accepted 5 November 2007


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