JXB Advance Access originally published online on March 7, 2008
Journal of Experimental Botany 2008 59(4):891-905; doi:10.1093/jxb/ern014
© 2008 The Author(s).
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RESEARCH PAPER |
Development of abiotic stress tolerance via bZIP-type transcription factor LIP19 in common wheat
1Laboratory of Plant Genetics, Graduate School of Agricultural Science, Kobe University, 1-1 Rokkodai-cho, Nada-ku, Kobe 657-8501, Japan
2Kihara Institute for Biological Research, Graduate School of Integrated Science, Yokohama City University, Totsuka-ku, Yokohama 244-0813, Japan
To whom correspondence should be addressed. E-mail: takumi{at}kobe-u.ac.jp
Cereal lip19 genes encoding bZIP-type transcription factors are assumed to play a regulatory role in gene expression during the cold acclimation process. However, no direct evidence shows an association of LIP19-type bZIPs with stress tolerance or activation of stress-responsive Cor/Lea genes. To understand the molecular basis of development of abiotic stress tolerance through the LIP19 transcription factor, a wheat lip19 homologue, Wlip19, was isolated and characterized. Wlip19 expression was activated by low temperature in seedlings and was higher in a freezing-tolerant cultivar than in a freezing-sensitive one. Wlip19 also responded to drought and exogenous ABA treatment. Wlip19-expressing transgenic tobacco showed a significant increase in abiotic stress tolerance, especially freezing tolerance. Expression of a GUS reporter gene under the control of promoter sequences of four wheat Cor/Lea genes, Wdhn13, Wrab17, Wrab18, and Wrab19, was enhanced by Wlip19 expression in wheat callus and tobacco plants. These results indicate that WLIP19 acts as a transcriptional regulator of Cor/Lea genes in the development of abiotic stress tolerance. Moreover, direct protein–protein interaction between WLIP19 and a wheat OBF1 homologue TaOBF1, another bZIP-type transcription factor, was observed, suggesting that this interaction is conserved in cereals.
Key words: Abiotic stress tolerance, bZIP protein, Cor/Lea genes, transgenic plants, Triticum aestivum L
* Present address: Plant Genome Research Unit, National Institute of Agrobiological Science, 2-1-2 Kannondai, Tsukuba, Ibaraki 305-8602, Japan.
Received 12 November 2007; Revised 3 January 2008 Accepted 7 January 2008
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