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JXB Advance Access originally published online on March 5, 2008
Journal of Experimental Botany 2008 59(4):927-937; doi:10.1093/jxb/ern033
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© 2008 The Author(s).
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)

RESEARCH PAPER

Comparative mapping of HKT genes in wheat, barley, and rice, key determinants of Na+ transport, and salt tolerance

Shaobai Huang *, Wolfgang Spielmeyer, Evans S. Lagudah and Rana Munns{dagger}

CSIRO Plant Industry, Canberra, Australian Capital Territory 2601, Australia

{dagger}To whom correspondence should be addressed: E-mail: rana.munns{at}csiro.au

Salt tolerance of plants depends on HKT transporters (High-affinity K+ Transporter), which mediate Na+-specific transport or Na+-K+ co-transport. Gene sequences closely related to rice HKT genes were isolated from hexaploid bread wheat (Triticum aestivum) or barley (Hordeum vulgare) for genomic DNA southern hybridization analysis. HKT gene sequences were mapped on chromosomal arms of wheat and barley using wheat chromosome substitution lines and barley–wheat chromosome addition lines. In addition, HKT gene members in the wild diploid wheat ancestors, T. monococcum (Am genome), T. urartu (Au genome), and Ae. tauschii (Dt genome) were investigated. Variation in copy number for individual HKT gene members was observed between the barley, wheat, and rice genomes, and between the different wheat genomes. HKT2;1/2-like, HKT2;3/4-like, HKT1;1/2-like, HKT1;3-like, HKT1;4-like, and HKT1;5-like genes were mapped to the wheat–barley chromosome groups 7, 7, 2, 6, 2, and 4, respectively. Chromosomal regions containing HKT genes were syntenic between wheat and rice except for the chromosome regions containing the HKT1;5-like gene. Potential roles of HKT genes in Na+ transport in rice, wheat, and barley are discussed. Determination of the chromosome locations of HKT genes provides a framework for future physiological and genetic studies investigating the relationships between HKT genes and salt tolerance in wheat and barley.

Key words: Barley, comparative mapping, HKT, rice, salt tolerance, sodium transport, wheat

* Present address: ARC Centre of Excellence in Plant Energy Biology, The University of Western Australia, 35 Stirling Highway, Crawley, WA 6009, Australia.

Received 21 September 2007; Revised 7 January 2008 Accepted 9 January 2008


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