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JXB Advance Access originally published online on April 4, 2008
Journal of Experimental Botany 2008 59(6):1375-1381; doi:10.1093/jxb/ern068
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© The Author [2008]. Published by Oxford University Press [on behalf of the Society for Experimental Biology]. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

RESEARCH PAPER

Expression of the nuclear gene TaFAd is under mitochondrial retrograde regulation in anthers of male sterile wheat plants with timopheevii cytoplasm

Pei Xu1, Yuwen Yang1, Zhengzhi Zhang1, Weihua Chen2, Caiqin Zhang1, Lixia Zhang1, Sixiang Zou2 and Zhengqiang Ma1,*

1The Applied Plant Genomics Laboratory, Crop Genomics and Bioinformatics Center & National Key Lab of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Jiangsu 210095, China
2College of Veterinary Medicine, Nanjing Agricultural University, Jiangsu 210095, China

* To whom correspondence should be addressed. E-mail: zqm2{at}njau.edu.cn

Alterations of mitochondrial-encoded subunits of the FoF1-ATP synthase are frequently associated with cytoplasmic male sterility (CMS) in plants; however, little is known about the relationship of the nuclear encoded subunits of this enzyme with CMS. In the present study, the full cDNA of the gene TaFAd that encodes the putative FAd subunit of the FoF1-ATP synthase was isolated from the wheat (Triticum aestivum) fertility restorer ‘2114’ for timopheevii cytoplasm-based CMS. The deduced 238 amino acid polypeptide is highly similar to its counterparts in dicots and other monocots but has low homology to its mammalian equivalents. TaFAd is a single copy gene in wheat and maps to the short arm of the group 6 chromosomes. Transient expression of the TaFAd–GFP fusion in onion epidermal cells demonstrated TaFAd's mitochondrial location. TaFAd was expressed abundantly in stem, leaf, anther, and ovary tissues of 2114. Nevertheless, its expression was repressed in anthers of CMS plants with timopheevii cytoplasm. Genic male sterility did not affect its expression in anthers. The expression of the nuclear gene encoding the 20 kDa subunit of Fo was down-regulated in a manner similar to TaFAd in the T-CMS anthers while that of genes encoding the 6 kDa subunit of Fo and the {gamma} subunit of F1 was unaffected. These observations implied that TaFAd is under mitochondrial retrograde regulation in the anthers of CMS plants with timopheevii cytoplasm.

Key words: CMS, FAd subunit, FoF1-ATP synthase, retrograde regulation, wheat

Received 8 October 2007; Revised 9 January 2008 Accepted 28 January 2008


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