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JXB Advance Access originally published online on February 9, 2009
Journal of Experimental Botany 2009 60(4):1273-1288; doi:10.1093/jxb/erp008
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© 2009 The Author(s).
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)

RESEARCH PAPER

Potato (Solanum tuberosum L.) tuber ageing induces changes in the proteome and antioxidants associated with the sprouting pattern

Pierre Delaplace1,*, Marie-Laure Fauconnier1, Kjell Sergeant2, Jean-François Dierick3, Mouhssin Oufir2, Froukje van der Wal4, Antoine H. P. America4, Jenny Renaut2, Jean-François Hausman2 and Patrick du Jardin1

1Gembloux Agricultural University, Plant Biology Unit, Avenue de la Faculté d'Agronomie 2A, 5030 Gembloux, Belgium
2Centre de Recherche Public Gabriel Lippmann, Environment and Agro-Biotechnologies Department (EVA), Rue du Brill 41, L-4422 Belvaux, Luxembourg
3BioVallée, Proteomics Unit, Rue Adrienne Bolland 8, 6041 Charleroi, Belgium
4Plant Research International, BU Bioscience, Wageningen University and Research Centre, PO Box 16, 6700AA, Wageningen, The Netherlands

* To whom correspondence should be addressed. E-mail: delaplace.p{at}fsagx.ac.be

During post-harvest storage, potato tubers age as they undergo an evolution of their physiological state influencing their sprouting pattern. In the present study, physiological and biochemical approaches were combined to provide new insights on potato (Solanum tuberosum L. cv. Désirée) tuber ageing. An increase in the physiological age index (PAI) value from 0.14 to 0.83 occurred during storage at 4 °C over 270 d. Using this reference frame, a proteomic approach was followed based on two-dimensional electrophoresis. In the experimental conditions of this study, a marked proteolysis of patatin occurred after the PAI reached a value of 0.6. In parallel, several glycolytic enzymes were up-regulated and cellular components influencing protein conformation and the response to stress were altered. The equilibrium between the 20S and 26S forms of the proteasome was modified, the 20S form that recycles oxidized proteins being up-regulated. Two proteins belonging to the cytoskeleton were also differentially expressed during ageing. As most of these changes are also observed in an oxidative stress context, an approach focused on antioxidant compounds and enzymes as well as oxidative damage on polyunsaturated fatty acids and proteins was conducted. All the changes observed during ageing seemed to allow the potato tubers to maintain their radical scavenging activity until the end of the storage period as no accumulation of oxidative damage was observed. These data are interpreted considering the impact of reactive oxygen species on the development and the behaviour of other plant systems undergoing ageing or senescence processes.

Key words: Ascorbate, carbonyl, glutathione, oxylipin, phenolic compounds, physiological age index (PAI), radical scavenging activity, reactive oxygen species, sprouting pattern, two-dimensional electrophoresis

Received 17 September 2008; Revised 18 December 2008 Accepted 7 January 2009


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