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JXB Advance Access originally published online on April 2, 2009
Journal of Experimental Botany 2009 60(6):1729-1742; doi:10.1093/jxb/erp053
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© 2009 The Author(s).
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)

RESEARCH PAPER

Influence of Arabidopsis thaliana accessions on rhizobacterial communities and natural variation in root exudates

Shirley A. Micallef, Michael P. Shiaris and Adán Colón-Carmona*

Department of Biology, University of Massachusetts Boston, 100 Morrissey Boulevard, Boston, MA 02125, USA

* To whom correspondence should be addressed: E-mail: adan.colon-carmona{at}umb.edu

Plant species is considered to be one of the most important factors in shaping rhizobacterial communities, but specific plant–microbe interactions in the rhizosphere are still not fully understood. Arabidopsis thaliana, for which a large number of naturally occurring ecotype accessions exist, lacks mycorrhizal associations and is hence an ideal model for rhizobacterial studies. Eight Arabidopsis accessions were found to exert a marked selective influence on bacteria associated with their roots, as determined by terminal-restriction fragment length polymorphism (T-RFLP) and ribosomal intergenic spacer analysis (RISA). Community differences in species composition and relative abundance were both significant (P <0.001). The eight distinct and reproducible accession-dependent community profiles also differed from control bulk soil. Root exudates of these variants were analysed by high performance liquid chromatography (HPLC) to try to establish whether the unique rhizobacterial assemblages among accessions could be attributed to plant-regulated chemical changes in the rhizosphere. Natural variation in root exudation patterns was clearly exhibited, suggesting that differences in exudation patterns among accessions could be influencing bacterial assemblages. Other factors such as root system architecture are also probably involved. Finally, to investigate the Arabidopsis rhizosphere further, the phylogenetic diversity of rhizobacteria from accession Cvi-0 is described.

Key words: Arabidopsis accessions, multi-dimensional scaling, natural variation, rhizobacterial communities, rhizosphere, root exudates

Received 9 December 2008; Accepted 3 February 2009


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