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Journal of Experimental Botany 2009 60(7):2093-2106; doi:10.1093/jxb/erp085
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© 2009 The Author(s).
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)

RESEARCH PAPER

Stilbene synthase gene transfer caused alterations in the phenylpropanoid metabolism of transgenic strawberry (Fragariaxananassa)

Kati Hanhineva1 *,{dagger}, Harri Kokko1, Henri Siljanen1, Ilana Rogachev2, Asaph Aharoni2 and Sirpa O. Kärenlampi1

1Department of Biosciences, University of Kuopio, PO Box 1627, 70211 Kuopio, Finland
2Weizmann Institute of Science, PO Box 26, 76100 Rehovot, Israel

{dagger} To whom correspondence should be addressed. E-mail: kati.hanhineva{at}uku.fi

The gene encoding stilbene synthase is frequently used to modify plant secondary metabolism with the aim of producing the self-defence phytoalexin resveratrol. In this study, strawberry (Fragariaxananassa) was transformed with the NS-Vitis3 gene encoding stilbene synthase from frost grape (Vitis riparia) under the control of the cauliflower mosaic virus 35S and the floral filament-specific fil1 promoters. Changes in leaf metabolites were investigated with UPLC-qTOF-MS (ultra performance liquid chromatography-quadrupole time of flight mass spectrometry) profiling, and increased accumulation of cinnamate, coumarate, and ferulate derivatives concomitantly with a decrease in the levels of flavonols was observed, while the anticipated resveratrol or its derivatives were not detected. The changed metabolite profile suggested that chalcone synthase was down-regulated by the genetic modification; this was verified by decreased chalcone synthase transcript levels. Changes in the levels of phenolic compounds led to increased susceptibility of the transgenic strawberry to grey mould fungus.

Key words: Genetic engineering, LC-MS, metabolite profiling, metabolomics, phenolic compounds, strawberry, stilbene synthase

* Present address: Department of Clinical Nutrition/Food and Health Research Centre, University of Kuopio, PO Box 1627, 70211 Kuopio, Finland

Received 4 December 2008; Revised 13 February 2009 Accepted 25 February 2009


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