Skip Navigation



JXB Advance Access published online on March 3, 2003
Journal of Experimental Botany, doi:10.1093/jxb/erg114
© 2003 by Oxford University Press
This Article
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
54/385/1133    most recent
erg114v1
Right arrow E-letters: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when E-letters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Rose, A.
Right arrow Articles by Meier, I.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Rose, A.
Right arrow Articles by Meier, I.
Agricola
Right arrow Articles by Rose, A.
Right arrow Articles by Meier, I.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Received August 19, 2002; accepted November 30, 2002
© 2003 Society for Experimental Biology

RESEARCH PAPER

A novel alpha-helical protein, specific to and highly conserved in plants, is associated with the nuclear matrix fraction

Annkatrin Rose 1, Frank Gindullis 1, Iris Meier 1*

1 Plant Biotechnology Center and Department of Plant Biology, Ohio State University, Columbus, OH 43210, USA

* To whom correspondence should be addressed. E-mail: meier.56{at}osu.edu.


  Abstract

A cDNA for a novel plant protein was isolated from tomato. Nuclear Matrix Protein 1 (NMP1) is a ubiquitously expressed 36 kDa protein, which has no homologues in animals and fungi, but is highly conserved among flowering and non-flowering plants, including gymnosperms, moss, and the liverwort Marchantia polymorpha. NMP1 is predominantly {alpha}-helical with multiple stretches of short amphipathic regions. Cell fractionation, immunofluorescence, and GFP localization experiments showed that NMP1 is located both in the cytoplasm and nucleus and that the nuclear fraction is associated with the nuclear matrix. NMP1 is a candidate for a plant-specific structural protein with a function both in the nucleus and cytoplasm.

Key words: Amphipathic alpha helix, GFP, nuclear matrix, tomato.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J Exp BotHome page
R. Samaniego, S. Y. Jeong, C. de la Torre, I. Meier, and S. M. Diaz de la Espina
CK2 phosphorylation weakens 90 kDa MFP1 association to the nuclear matrix in Allium cepa
J. Exp. Bot., January 1, 2006; 57(1): 113 - 124.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.