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JXB Advance Access published online on March 3, 2003
Journal of Experimental Botany, doi:10.1093/jxb/erg123
© 2003 by Oxford University Press
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Received September 13, 2002; accepted January 2, 2003
© 2003 Society for Experimental Biology

RESEARCH PAPER

A jasmonate-responsive element within the A. thaliana vsp1 promoter

F. Guerineau 1*, M. Benjdia 2, D. X. Zhou 3

1 Université de Picardie Jules Verne, 33 rue St Leu, F-80039 Amiens, France
2 ZMBP, Plant Physiology, Auf der Morgenstelle 1, Universität Tubingen, D-72076, Tubingen, Germany
3 Institut de biotechnologie des plantes, Université de Paris-sud, F-91405 Orsay, France

* To whom correspondence should be addressed. E-mail: francois.guerineau{at}sc.u-picardie.fr.


  Abstract

The vsp1 gene of Arabidopsis thaliana encodes a storage protein that accumulates in vegetative organs. Transgenic plants expressing a vsp1 promoter-gus ({beta}-glucuronidase) gene fusion were found to contain high GUS activity when challenged with jasmonate, a volatile plant hormone. The induction of vsp1-gus expression by jasmonate could be measured in tobacco mesophyll protoplasts, after transient expression. A number of deletions were operated in the vsp1 promoter in order to locate its jasmonate-responsive element. A 41 bp sequence taken approximately 150 bp upstream of the vsp1 TATA box could confer jasmonate responsiveness upon a short CaMV 35S promoter. Whereas the deletion of a CAAT box-like element within the 41 bp sequence did not affect promoter activity, mutation of a short palindromic motif completely abolished jasmonate responsiveness. This motif shows no homology with the jasmonate-responsive elements of other promoters.

Key words: Arabidopsis thaliana, gene expression, jasmonate, promoter, vegetative storage protein.


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