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JXB Advance Access published online on March 31, 2003
Journal of Experimental Botany, doi:10.1093/jxb/erg147
© 2003 by Oxford University Press
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Received August 12, 2002; accepted January 18, 2003
© 2003 Society for Experimental Biology

RESEARCH PAPER

Quick and reversible inhibition of soybean root nodule growth by nitrate involves a decrease in sucrose supply to nodules

Hiroyuki Fujikake 1, Akihiko Yamazaki 1, Norikuni Ohtake 1, Kuni Sueyoshi 1, Shinpei Matsuhashi 2, Takehito Ito 2, Chizuko Mizuniwa 2, Tamikazu Kume 2, Shoji Hashimoto 2, Noriko-Shigeta Ishioka 3, Satoshi Watanabe 3, Akihiko Osa 3, Toshiaki Sekine 3, Hiroshi Uchida 4, Atsunori Tsuji 4, Takuji Ohyama 1*

1 Faculty of Agriculture, Niigata University, 2-8050 Ikarashi, Niigata, 950-2181, Japan
2 Takasaki Radiation Chemistry Research Establishment, Japan Atomic Energy Research Institute, Gunma 370-1207, Japan
3 Department of Radioisotopes, Japan Atomic Energy Research Institute, Gunma 370-1207, Japan
4 Central Research Laboratory, Hamamatsu Photonics Co., Shizuoka 434-0041, Japan

* To whom correspondence should be addressed. E-mail: ohyama{at}agr.niigata-u.ac.jp.


  Abstract

The upper part of a nodulated soybean root hydroponically cultured in a glass bottle was monitored using a computer microscope under controlled environmental conditions, and the diameter of individual nodules was measured from 10-24 d after planting. The diameter of a root nodule attached to the primary root increased from 1 mm to 6 mm for 2 weeks under nitrogen-free conditions. The increase in diameter of the nodules was almost completely stopped after 1 d of supplying 5 mM nitrate, and was due to the cessation of nodule cell expansion. However, nodule growth quickly returned to the normal growth rate following withdrawal of nitrate from the solution. The reversible depression of nodule growth by nitrate was similar to the restriction of photoassimilate supply by continuous dark treatment for 2 d followed by normal light/dark conditions. In addition, the inhibitory effect of nitrate was partially alleviated by the addition of 3% (w/v) sucrose to the medium. Plant leaves were exposed to 11C or 14C-labelled carbon dioxide to investigate the effects of 5 mM nitrate on the translocation and distribution of photosynthates to nodules and roots. Supplying 5 mM nitrate stimulated the translocation rate and the distribution of labelled C in nitrate-fed parts of the roots. However, the 14C partitioning to nodules decreased from 9% to 4% of total 14C under conditions of 5 mM nitrate supply. These results indicate that the decrease in photoassimilate supply to nodules may be involved in the quick and reversible nitrate inhibition of soybean nodule growth.

Key words: Nitrate inhibition, nodule growth, PETIS, soybean.


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