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JXB Advance Access published online on April 11, 2003
Journal of Experimental Botany, doi:10.1093/jxb/erg160
© 2003 by Oxford University Press
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Received September 3, 2002; accepted February 26, 2003
© 2003 Society for Experimental Biology

RESEARCH PAPER

Vacuolar system distribution in Arabidopsis tissues, visualized using GFP fusion proteins

Ricardo Flückiger 1, Monica De Caroli 2, Gabriella Piro 2, Giuseppe Dalessandro 2, Jean-Marc Neuhaus 1, Gian-Pietro Di Sansebastiano 2*

1 Laboratoire de Biochimie, Institute de Botanique, Université de Neuchâtel, rue Emile-Argand 9, CP 2, CH-2007 Neuchâtel 7, Switzerland
2 Laboratorio di Botanica, Di.S.Te.B.A., Università di Lecce, via pro.le Lecce-Monteroni, I-73100 Lecce, Italy

* To whom correspondence should be addressed. E-mail: gp.disansebastiano{at}unile.it.


  Abstract

Green fluorescent protein (GFP) allows the direct visualization of gene expression and the subcellular localization of fusion proteins in living cells. The localization of different GFP fusion proteins in the secretory system was studied in stably transformed Arabidopsis plants cv. Wassilewskaja. Secreted GFP (SGFP) and GFP retained in the ER (GFP-KDEL) confirmed patterns already known, but two vacuolar GFPs (GFP-Chi and Aleu-GFP) labelled the Arabidopsis vacuolar system for the first time, the organization of which appears to depend on cell differentiation. GFP stability in the vacuoles may depend on pH or degradation, but these vacuolar markers can, nevertheless, be used as a tool for physiological studies making these plants suitable for mutagenesis and gene-tagging experiments.

Key words: Arabidopsis, development, GFP, soluble protein sorting, vacuole biogenesis.


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