JXB Advance Access published online on May 13, 2003
Journal of Experimental Botany, doi:10.1093/jxb/erg177
© 2003 by Oxford University Press
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1 Max Planck Institute for Molecular Plant Physiology, Department of Molecular Physiology, Am Muehlenberg 1, D-14476 Golm, Germany
* To whom correspondence should be addressed. E-mail: hesse{at}mpimp-golm.mpg.de.
With the aim of analysing the relative importance of sugar supply and nitrogen nutrition for the regulation of sulphate assimilation, the regulation of adenosine 5'-phosphosulphate reductase (APR), a key enzyme of sulphate reduction in plants, was studied. Glucose feeding experiments with Arabidopsis thaliana cultivated with and without a nitrogen source were performed. After a 38 h dark period, APR mRNA, protein, and enzymatic activity levels decreased dramatically in roots. The addition of 0.5% (w/v) glucose to the culture medium resulted in an increase of APR levels in roots (mRNA, protein and activity), comparable to those of plants kept under normal light conditions. Treatment of roots with D-sorbitol or D-mannitol did not increase APR activity, indicating that osmotic stress was not involved in APR regulation. The addition of O-acetyl-L-serine (OAS) also quickly and transiently increased APR levels (mRNA, protein, and activity). Feeding plants with a combination of glucose and OAS resulted in a more than additive induction of APR activity. Contrary to nitrate reductase, APR was also increased by glucose in N-deficient plants, indicating that this effect was independent of nitrate assimilation. [35S]-sulphate feeding experiments showed that the addition of glucose to dark-treated roots resulted in an increased incorporation of [35S] into thiols and proteins, which corresponded to the increased levels of APR activity. Under N-deficient conditions, glucose also increased thiol labelling, but did not increase the incorporation of label into proteins. These results demonstrate that (i) exogenously supplied glucose can replace the function of photoassimilates in roots; (ii) APR is subject to co-ordinated metabolic control by carbon metabolism; (iii) positive sugar signalling overrides negative signalling from nitrate assimilation in APR regulation. Furthermore, signals originating from nitrogen and carbon metabolism regulate APR synergistically.
© 2003 Society for Experimental Biology
RESEARCH PAPER
Effect of glucose on assimilatory sulphate reduction in Arabidopsis thaliana roots
2 University of Bern, Institute of Plant Sciences, Altenbergrain 21, 3013 Bern, Switzerland
3 University of Freiburg, Institute of Forest Botany and Tree Physiology, Georges-Köhler-Allee, Geb. 053/054, D-79085 Freiburg, Germany
Abstract 
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  L. Lejay, J. Wirth, M. Pervent, J. M.-F. Cross, P. Tillard, and A. Gojon Oxidative Pentose Phosphate Pathway-Dependent Sugar Sensing as a Mechanism for Regulation of Root Ion Transporters by Photosynthesis Plant Physiology, April 1, 2008; 146(4): 2036 - 2053. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Koprivova, K. A. North, and S. Kopriva Complex Signaling Network in Regulation of Adenosine 5'-Phosphosulfate Reductase by Salt Stress in Arabidopsis Roots Plant Physiology, March 1, 2008; 146(3): 1408 - 1420. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Durenkamp, L. J. De Kok, and S. Kopriva Adenosine 5'-phosphosulphate reductase is regulated differently in Allium cepa L. and Brassica oleracea L. upon exposure to H2S J. Exp. Bot., May 1, 2007; 58(7): 1571 - 1579. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. KOPRIVA Regulation of Sulfate Assimilation in Arabidopsis and Beyond Ann. Bot., April 1, 2006; 97(4): 479 - 495. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Maruyama-Nakashita, Y. Nakamura, T. Yamaya, and H. Takahashi Regulation of high-affinity sulphate transporters in plants: towards systematic analysis of sulphur signalling and regulation J. Exp. Bot., August 1, 2004; 55(404): 1843 - 1849. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Sirko, A. Blaszczyk, and F. Liszewska Overproduction of SAT and/or OASTL in transgenic plants: a survey of effects J. Exp. Bot., August 1, 2004; 55(404): 1881 - 1888. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Kopriva and H. Rennenberg Control of sulphate assimilation and glutathione synthesis: interaction with N and C metabolism J. Exp. Bot., August 1, 2004; 55(404): 1831 - 1842. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Hesse, V. Nikiforova, B. Gakiere, and R. Hoefgen Molecular analysis and control of cysteine biosynthesis: integration of nitrogen and sulphur metabolism J. Exp. Bot., June 1, 2004; 55(401): 1283 - 1292. [Abstract] [Full Text] [PDF]
|
|