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JXB Advance Access published online on June 18, 2003
Journal of Experimental Botany, doi:10.1093/jxb/erg201
© 2003 by Oxford University Press
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Received April 21, 2003; accepted April 25, 2003
© 2003 Society for Experimental Biology

GENE NOTE

OsSET1, a novel SET-domain-containing gene from rice

Yun-Kuan Liang 1, Ying Wang 1, Yong Zhang 1, Song-Gang Li 1, Xiao-Chun Lu 1, Hong Li 1, Cheng Zou 1, Zhi-Hong Xu 1, and Shu-Nong Bai 1*

1 PKU-Yale Joint Research Center of Agricultural and Plant Molecular Biology, National Key Laboratory of Protein Engineering and Plant Gene Engineering, College of Life Sciences, Peking University, 5 Yiheyuan Road, Beijing 100871, PR China

* To whom correspondence should be addressed. E-mail: shunongb{at}pku.edu.cn.


  Abstract

A novel SET-domain-containing gene OsSET1 was isolated from rice (Oryza sativa L.). Its deduced protein consists of 895 amino acids. OsSET1 has a high degree of structure similarity to other SET-domain-containing genes such as CLF in higher plants and E(z) in animals. RT-PCR showed that the gene expresses throughout the entire plant. A transient expression assay in onion epidermis revealed that the OsSET1 protein is localized in nuclei. Over-expression of the SET domain of OsSET1 in Arabidopsis resulted in altered shoot development at seedling stages.

Key words: Gene expression regulation, rice, OsSET1, SET-domain-containing genes.


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