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JXB Advance Access published online on June 18, 2003
Journal of Experimental Botany, doi:10.1093/jxb/erg203
© 2003 by Oxford University Press
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Received January 20, 2003; accepted April 29, 2003
© 2003 Society for Experimental Biology

GENE NOTE

Cloning and expression of a UDP-glucuronic acid decarboxylase gene in rice

Kiyoshi Suzuki 1, Yasuhiko Suzuki 2, and Shinichi Kitamura 1*

1 Graduate School of Agriculture and Biological Sciences, Osaka Prefecture University, Gakuen-cho 1-1, Sakai, Osaka 599-8531, Japan
2 Division of Pathology, Osaka Prefectural Institute of Public Health, Nakamichi 1-3-69, Higashinari-ku, Osaka, Osaka 537-0025, Japan

* To whom correspondence should be addressed. E-mail: skita{at}biochem.osakafu-u.ac.jp.


  Abstract

A cDNA fragment was cloned from rice immature seeds by the RT-PCR method. The deduced amino acid sequence of the cDNA showed a high degree of identity with UDP-D-glucuronic acid decarboxylase (UXS) from other plants and was most similar to the soluble UXS from Arabidopsis. The recombinant protein, expressed in an Escherichia coli system, catalysed the conversion of UDP-D-glucuronic acid to UDP-D-xylose, confirming that the gene encoded UXS. The uxs gene was expressed in mature, harvested rice seeds as well as in immature seeds 14 d post-anthesis, suggesting that the uxs gene is necessary at the beginning of the germination period. This is the first report of the cloning of the uxs gene from monocots.

Key words: Endosperm, gene expression, Oryza sativa cv. Nipponbare (monocots), UDP-glucuronic acid decarboxylase (UXS).


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