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JXB Advance Access published online on August 8, 2003
Journal of Experimental Botany, doi:10.1093/jxb/erg254
© 2003 by Oxford University Press
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Received April 3, 2003; accepted July 1, 2003
© 2003 Society for Experimental Biology

GENE NOTE

Isolation and characterization of a Brassica napus cDNA corresponding to a B-class floral development gene

Jeffrey D. Pylatuik 1, Donna L. Lindsay 1, Arthur R. Davis 1, and Peta C. Bonham-Smith 1*

1 Department of Biology, 112 Science Place, University of Saskatchewan, Saskatoon, Saskatchewan, S7N 5E2, Canada

* To whom correspondence should be addressed. E-mail: bonhamp{at}duke.usask.ca.


  Abstract

B-class floral homeotic genes are required for the proper formation and identity of petals and stamens in dicot flowers. A partial cDNA clone encoding a B-class gene, BnAP3 (Brassica napus APETALA3), was isolated from a B. napus cDNA library derived from young inflorescence meristems. The 5' region of the cDNA was retrieved by RACE. The deduced amino acid sequence of the full-length clone exhibited high similarity to APETALA3 of Arabidopsis thaliana and functionally homologous proteins from other species. 5' RACE and Southern analysis suggests that BnAP3 has multiple alleles in B. napus. Expression analysis assayed by RT-PCR shows that BnAP3 is expressed in floral tissues, as well as non-floral tissues such as root and bract. Transformation of wild-type A. thaliana and B. napus plants with BnAP3 under the control of a promoter specific to reproductive organs converts carpels to stamens, while the expression of this construct in A. thaliana plants mutant for AP3 restores the development of third-whorl stamens in addition to directing a carpel to stamen conversion in the fourth whorl.

Key words: Floral development, Brassica napus, BnAP3.


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