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JXB Advance Access published online on September 25, 2003
Journal of Experimental Botany, doi:10.1093/jxb/erg271
© 2003 by Oxford University Press
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Received March 17, 2003; accepted July 17, 2003
© 2003 Society for Experimental Biology

RESEARCH PAPER

Self-incompatibility (S) locus region of the mutated S6-haplotype of sour cherry (Prunus cerasus) contains a functional pollen S allele and a non-functional pistil S allele

Hisayo Yamane 1, Kazuo Ikeda 1, Nathanael R. Hauck 2, Amy F. Iezzoni 2, and Ryutaro Tao 1*

1 Laboratory of Pomology, Graduate School of Agriculture, Kyoto University, Kyoto 606-8502, Japan
2 Department of Horticulture, Michigan State University, East Lansing, MI 48824, USA

* To whom correspondence should be addressed. E-mail: rtao{at}kais.kyoto-u.ac.jp.


  Abstract

This study characterizes the S6m-haplotype, a mutated S6-haplotype with an altered HindIII cut site, of sour cherry (Prunus cerasus). Inheritance and pollination studies of S-haplotypes from reciprocal crosses between ‘Erdi Botermo' (EB; S4S6mSa) and ‘Rheinische Schattenmorelle' (RS; S6SaSbSc) revealed that the S6m-haplotype conferred unilateral incompatibility with a non-functional pistil component and a functional pollen component. Expression analyses of S6-RNase and SFB6, a candidate gene for pollen-S, in the S6m-haplotype showed that SFB6 was transcribed in EB pollen, but S6-RNase was not transcribed in EB styles. These results were consistent with data from the inheritance and pollination studies. Inverse PCR for the flanking regions of S6-RNase in the S6- and S6m-haplotypes revealed an approximately 2600 bp insertion present at approximately 800 bp upstream of the S6-RNase in the S6m-haplotype, which is responsible for the alternation of the HindIII cut site and a possible cause of inhibition of the transcription of S6-RNase. SFB6 was present downstream of S6-RNase in both the S6- and S6m-haplotypes and expressed in the same way, supporting the idea that SFB is a good candidate for pollen-S in Prunus.

Key words: Pistil determinant, pollen determinant, polyploidy, Prunus avium, Rosaceae, SFB, S-RNase.


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