Skip Navigation



JXB Advance Access published online on January 30, 2004
Journal of Experimental Botany, doi:10.1093/jxb/erh055
© 2004 by Oxford University Press
This Article
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
55/397/547    most recent
erh055v1
Right arrow E-letters: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when E-letters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Yau, C. P.
Right arrow Articles by Yip, W. K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yau, C. P.
Right arrow Articles by Yip, W. K.
Agricola
Right arrow Articles by Yau, C. P.
Right arrow Articles by Yip, W. K.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Received July 9, 2003; accepted November 3, 2003
© 2004 Society for Experimental Biology

RESEARCH PAPER

Differential expression of three genes encoding an ethylene receptor in rice during development, and in response to indole-3-acetic acid and silver ions

Chi Ping Yau 1, Lingjian Wang 1, Manda Yu 1, Sze Yong Zee 1, and Wing Kin Yip 1*

1 Department of Botany, University of Hong Kong, Pokfulam Road, Hong Kong

* To whom correspondence should be addressed. E-mail: wkyip{at}hkucc.hku.hk.


  Abstract

Five ethylene receptor genes, OS-ERS1, OS-ERS2, OS-ETR2, OS-ETR3, and OS-ETR4 were isolated and characterized from rice. The genomic structure of OS-ERS1 and OS-ERS2 revealed that the introns within the coding sequences occurred in conserved positions to those of At-ETR1 and At-ERS1, whereas each of the OS-ETR2, OS-ETR3, and OS-ETR4 genes contained 1 intron within its coding region located at a position equivalent to those of At-ERS2, At-ETR2, and At-EIN4. Deduced amino acid sequences of OS-ERS1, OS-ERS2, OS-ETR2, OS-ETR3, and OS-ETR4 showed that they exhibited significant homology to the prokaryotic two-component signal transducer and a wide range of ethylene receptors in a variety of plant species. Northern analysis revealed that the level of OS-ETR2 mRNA was markedly elevated either by the exogenous application of IAA or by ethylene treatment in young etiolated rice seedlings, whereas the OS-ERS1 transcript level was only slightly induced under the same experimental conditions. Pretreatment with silver prevented IAA-induced and ethylene-induced accumulation of both mRNAs (OS-ERS1 and OS-ETR2). However, the abundance of OS-ERS2 mRNA was shown to be down-regulated by both IAA and ethylene treatments, indicating that it was not positively regulated by ethylene. Analysis of the expression of the three ethylene receptor genes in different tissues of rice has unravelled their corresponding tissue-specificity in which OS-ERS1 was constitutively expressed in considerable amounts in all tissues studied, while OS-ERS2 and OS-ETR2 exhibited differential expression patterns in different tissues of rice. Moreover, higher levels of these three mRNAs were commonly observed in anthers when compared with their corresponding levels in other tissues, suggesting the important role played by ethylene involved in the regulation of pollen development in rice. Among the five ethylene receptor genes, the expression levels of both OS-ETR3 and OS-ETR4 were too low to be detected by the northern blot analysis. Results from RT-PCR illustrated that both mRNAs were present in young green rice seedlings and anthers.

Key words: Auxin, differential expression, ethylene, ethylene receptors, rice.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Plant Cell PhysiolHome page
T. Chen, J. Liu, G. Lei, Y.-F. Liu, Z.-G. Li, J.-J. Tao, Y.-J. Hao, Y.-R. Cao, Q. Lin, W.-K. Zhang, et al.
Effects of Tobacco Ethylene Receptor Mutations on Receptor Kinase Activity, Plant Growth and Stress Responses
Plant Cell Physiol., September 1, 2009; 50(9): 1636 - 1650.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
H. Wuriyanghan, B. Zhang, W.-H. Cao, B. Ma, G. Lei, Y.-F. Liu, W. Wei, H.-J. Wu, L.-J. Chen, H.-W. Chen, et al.
The Ethylene Receptor ETR2 Delays Floral Transition and Affects Starch Accumulation in Rice
PLANT CELL, May 1, 2009; 21(5): 1473 - 1494.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
X.-r. Yin, K.-s. Chen, A. C. Allan, R.-m. Wu, B. Zhang, N. Lallu, and I. B. Ferguson
Ethylene-induced modulation of genes associated with the ethylene signalling pathway in ripening kiwifruit
J. Exp. Bot., May 1, 2008; 59(8): 2097 - 2108.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
G. E. Schaller, K. Doi, I. Hwang, J. J. Kieber, J. P. Khurana, N. Kurata, T. Mizuno, A. Pareek, S.-H. Shiu, P. Wu, et al.
Nomenclature for Two-Component Signaling Elements of Rice
Plant Physiology, February 1, 2007; 143(2): 555 - 557.
[Full Text] [PDF]

Home page
 Plant Physiol.Home page
A. Pareek, A. Singh, M. Kumar, H. R. Kushwaha, A. M. Lynn, and S. L. Singla-Pareek
Whole-Genome Analysis of Oryza sativa Reveals Similar Architecture of Two-Component Signaling Machinery with Arabidopsis
Plant Physiology, October 1, 2006; 142(2): 380 - 397.
[Abstract] [Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
Y.-F. CHEN, N. ETHERIDGE, and G. E. SCHALLER
Ethylene Signal Transduction
Ann. Bot., May 1, 2005; 95(6): 901 - 915.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.