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JXB Advance Access published online on January 30, 2004
Journal of Experimental Botany, doi:10.1093/jxb/erh062
© 2004 by Oxford University Press
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Received September 4, 2003; accepted November 10, 2003
© 2004 Society for Experimental Biology

Research paper

GFP-labelled Rubisco and aspartate aminotransferase are present in plastid stromules and traffic between plastids

Ernest Y. Kwok 1 and Maureen R. Hanson 1*

1 Department of Molecular Biology and Genetics, 321 Biotechnology Building, Cornell University, Ithaca, NY 14853, USA

* To whom correspondence should be addressed. E-mail: mrh5{at}cornell.edu.


  Abstract

Plastid stromules are membrane-bound protrusions of the plastid envelope that contain soluble stroma. Stromules are often found connecting plastids within a cell and fluorescence recovery after photobleaching (FRAP) experiments have demonstrated that green fluorescent protein (GFP) can move between plastids via these connections. In this report, the ability of endogenous plastid proteins to travel through stromules was investigated. The motility of GFP-labelled plastid aspartate aminotransferase and the Rubisco small subunit was studied in stromules by FRAP. Both fusion proteins assemble into protein complexes that appear to behave similarly to their endogenous counterparts. In addition, both enzymes are capable of trafficking between plastids via stromules.

Key words: Aspartate aminotransferase, chloroplast, FRAP, photobleaching, plastid, protein trafficking, Rubisco, stromule.


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