Skip Navigation



JXB Advance Access published online on February 13, 2004
Journal of Experimental Botany, doi:10.1093/jxb/erh079
© 2004 by Oxford University Press
This Article
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
55/397/623    most recent
erh079v1
Right arrow E-letters: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when E-letters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Bari, R.
Right arrow Articles by Peterhänsel, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bari, R.
Right arrow Articles by Peterhänsel, C.
Agricola
Right arrow Articles by Bari, R.
Right arrow Articles by Peterhänsel, C.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Received September 12, 2003; accepted November 28, 2003
© 2004 Society for Experimental Biology

Research paper

A glycolate dehydrogenase in the mitochondria of Arabidopsis thaliana

Rafijul Bari 1, Rashad Kebeish 1, Rainer Kalamajka 1, Thomas Rademacher 2, and Christoph Peterhänsel 1*

1 Institute for Biology I, Aachen University, Worringer Weg 1, D-52056 Aachen, Germany
2 Fraunhofer Institute of Molecular Biology and Applied Ecology, D-52074 Aachen, Germany

* To whom correspondence should be addressed. E-mail: cp{at}bio1.rwth-aachen.de.


  Abstract

The fixation of molecular O2 by the oxygenase activity of Rubisco leads to the formation of phosphoglycolate in the chloroplast that is further metabolized in the process of photorespiration. The initial step of this pathway is the oxidation of glycolate to glyoxylate. Whereas in higher plants this reaction takes place in peroxisomes and is dependent on oxygen as a co-factor, most algae oxidize glycolate in the mitochondria using organic co-factors. The identification and characterization of a novel glycolate dehydrogenase in Arabidopsis thaliana is reported here. The enzyme is dependent on organic co-factors and resembles algal glycolate dehydrogenases in its enzymatic properties. Mutants of E. coli incapable of glycolate oxidation can be complemented by overexpression of the Arabidopsis open reading frame. The corresponding RNA accumulates preferentially in illuminated leaves, but was also found in other tissues investigated. A fusion of the N-terminal part of the Arabidopsis glycolate dehydrogenase to red fluorescent protein accumulates in mitochondria when overexpressed in the homologous system. Based on these results it is proposed that the basic photorespiratory system of algae is conserved in higher plants.

Key words: Arabidopsis thaliana, dehydrogenase, glycolate, mitochondrion, photorespiration.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Mol. Cell. ProteomicsHome page
C. P. Lee, H. Eubel, N. O'Toole, and A. H. Millar
Heterogeneity of the Mitochondrial Proteome for Photosynthetic and Non-photosynthetic Arabidopsis Metabolism
Mol. Cell. Proteomics, July 1, 2008; 7(7): 1297 - 1316.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
A. Nunes-Nesi, R. Sulpice, Y. Gibon, and A. R. Fernie
The enigmatic contribution of mitochondrial function in photosynthesis
J. Exp. Bot., May 1, 2008; 59(7): 1675 - 1684.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
M. Niessen, K. Thiruveedhi, R. Rosenkranz, R. Kebeish, H.-J. Hirsch, F. Kreuzaler, and C. Peterhansel
Mitochondrial glycolate oxidation contributes to photorespiration in higher plants
J. Exp. Bot., July 1, 2007; 58(10): 2709 - 2715.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
D. R. Luesse, S. L. DeBlasio, and R. P. Hangarter
Plastid Movement Impaired 2, a New Gene Involved in Normal Blue-Light-Induced Chloroplast Movements in Arabidopsis
Plant Physiology, August 1, 2006; 141(4): 1328 - 1337.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
H.-W. Xu, X.-M. Ji, Z.-H. He, W.-P. Shi, G.-H. Zhu, J.-K. Niu, B.-S. Li, and X.-X. Peng
Oxalate accumulation and regulation is independent of glycolate oxidase in rice leaves
J. Exp. Bot., June 1, 2006; 57(9): 1899 - 1908.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
C. L. Tavano, A. M. Podevels, and T. J. Donohue
Identification of Genes Required for Recycling Reducing Power during Photosynthetic Growth
J. Bacteriol., August 1, 2005; 187(15): 5249 - 5258.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.