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JXB Advance Access published online on February 27, 2004
Journal of Experimental Botany, doi:10.1093/jxb/erh096
© 2004 by Oxford University Press
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Received July 25, 2003; accepted December 16, 2003
© 2004 Society for Experimental Biology

GENE NOTE

Cloning and expression of cytosolic phosphoglycerate kinase from pea (Pisum sativum L.)

David C. Brice 1, John A. Bryant 1*, Gerardas Dambrauskas 1, Suzanne C. Drury 1, and Jennifer A. Littlechild 1

1 Department of Biological Sciences, University of Exeter, Exeter EX4 4PS, UK

* To whom correspondence should be addressed. E-mail: J.A.Bryant{at}ex.ac.uk.


  Abstract

In common with several other respiratory and photosynthetic enzymes, a sub-population of cytosolic phosphoglycerate kinase (PGK) occurs in the nucleus in pea leaves and shoots. The full-length cDNA encoding pea cytosolic PGK has been cloned and sequenced, revealing not only the PGK ‘signature’ but also a nuclear localization signal (NLS). A translational fusion of PGK and GFP was used to transform tobacco BY-2 cells resulting in GFP locating to the cell nuclei.

Key words: GFP, moonlighting proteins, nucleus, pea, phosphoglycerate kinase, Pisum sativum.


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