Skip Navigation



JXB Advance Access published online on March 12, 2004
Journal of Experimental Botany, doi:10.1093/jxb/erh102
© 2004 by Oxford University Press
This Article
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
55/398/909    most recent
erh102v1
Right arrow E-letters: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when E-letters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Castillejo, C.
Right arrow Articles by Valpuesta, V.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Castillejo, C.
Right arrow Articles by Valpuesta, V.
Agricola
Right arrow Articles by Castillejo, C.
Right arrow Articles by Valpuesta, V.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Received September 10, 2003; accepted January 20, 2004
© 2004 Society for Experimental Biology

RESEARCH PAPER

Pectin esterase gene family in strawberry fruit: study of FaPE1, a ripening-specific isoform

Cristina Castillejo 1, José Ignacio de la Fuente 1, Pietro Iannetta 2, Miguel Ángel Botella 1, and Victoriano Valpuesta 1*

1 Departamento de Biología Molecular y Bioquímica, Facultad de Ciencias, Universidad de Málaga,Campus de Teatinos, E-29071 Málaga, Spain
2 Ecosystem Management and Biotechnology, Scottish Crop Research Institute, Dundee DD2 4DN, UK

* To whom correspondence should be addressed. E-mail: valpuesta{at}uma.es.


  Abstract

Pectin esterases (PE, EC 3.1.1.11) catalyse the demethylation of pectin. As a result of its activity, structural interactions among cell wall components during cell wall turnover and loosening are affected. In plants, PEs are typically encoded by a gene family. This family has been studied in strawberry (Fragariaxananassa Duch.) in order to investigate the role of distinct PE genes during fruit ripening and senescence. By a combination of a PCR-based library screening and RT-PCR four different strawberry PE cDNAs, termed FaPE1 to FaPE4, have been isolated. Differential expression of each FaPE gene in various organs and during fruit development was revealed by northern blot. FaPE1 is specifically expressed in fruit, showing an increasing expression during the ripening process up to a maximum in the turning stage. Concerning hormone regulation, auxin treatment increased FaPE1 mRNA levels in green fruit, whereas exogenous ethylene decreased FaPE1 mRNA levels in ripe and senescing fruits. It is proposed that this repression of FaPE1 expression could be involved in textural changes occurring during fruit senescence.

Key words: Auxin, cell wall, ethylene, fruit ripening, pectin esterase, strawberry.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Plant Physiol.Home page
A. Fait, K. Hanhineva, R. Beleggia, N. Dai, I. Rogachev, V. J. Nikiforova, A. R. Fernie, and A. Aharoni
Reconfiguration of the Achene and Receptacle Metabolic Networks during Strawberry Fruit Development
Plant Physiology, October 1, 2008; 148(2): 730 - 750.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
P. Abbal, M. Pradal, F.-X. Sauvage, P. Chatelet, S. Paillard, A. Canaguier, A.-F. Adam-Blondon, and C. Tesniere
Molecular characterization and expression analysis of the Rop GTPase family in Vitis vinifera
J. Exp. Bot., July 1, 2007; 58(10): 2641 - 2652.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
J. I. de la Fuente, I. Amaya, C. Castillejo, J. F. Sanchez-Sevilla, M. A. Quesada, M. A. Botella, and V. Valpuesta
The strawberry gene FaGAST affects plant growth through inhibition of cell elongation
J. Exp. Bot., July 1, 2006; 57(10): 2401 - 2411.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
L. Trainotti, A. Pavanello, and G. Casadoro
Different ethylene receptors show an increased expression during the ripening of strawberries: does such an increment imply a role for ethylene in the ripening of these non-climacteric fruits?
J. Exp. Bot., August 1, 2005; 56(418): 2037 - 2046.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
M. Martinez, Z. Abraham, M. Gambardella, M. Echaide, P. Carbonero, and I. Diaz
The strawberry gene Cyf1 encodes a phytocystatin with antifungal properties
J. Exp. Bot., July 1, 2005; 56(417): 1821 - 1829.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
A. Wawrzynska, M. Lewandowska, M. J. Hawkesford, and A. Sirko
Using a suppression subtractive library-based approach to identify tobacco genes regulated in response to short-term sulphur deficit
J. Exp. Bot., June 1, 2005; 56(416): 1575 - 1590.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
W. G. van Doorn and E. J. Woltering
Senescence and programmed cell death: substance or semantics?
J. Exp. Bot., October 1, 2004; 55(406): 2147 - 2153.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.