Skip Navigation



JXB Advance Access published online on March 26, 2004
Journal of Experimental Botany, doi:10.1093/jxb/erh127
© 2004 by Oxford University Press
This Article
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
55/399/1003    most recent
erh127v1
Right arrow E-letters: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when E-letters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Zhao, J.
Right arrow Articles by Sakai, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zhao, J.
Right arrow Articles by Sakai, K.
Agricola
Right arrow Articles by Zhao, J.
Right arrow Articles by Sakai, K.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Received November 25, 2003; accepted February 10, 2004
© 2004 Society for Experimental Biology

RESEARCH PAPER

Jasmonate and ethylene signalling and their interaction are integral parts of the elicitor signalling pathway leading to {beta}-thujaplicin biosynthesis in Cupressus lusitanica cell cultures

Jian Zhao 1*, Shao-Hui Zheng 2, Koki Fujita 3, and Kokki Sakai 3

1 Laboratory of Forest Chemistry and Biochemistry, Faculty of Agriculture, Kyushu University, Fukuoka 812-8581, Japan; Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China
2 Laboratory of Crop Science, Faculty of Agriculture, Kyushu University, Fukuoka 812-8581 Japan
3 Laboratory of Forest Chemistry and Biochemistry, Faculty of Agriculture, Kyushu University, Fukuoka 812-8581, Japan

* To whom correspondence should be addressed. E-mail: jianzhao{at}ksu.edu.


  Abstract

Roles of jasmonate and ethylene signalling and their interaction in yeast elicitor-induced biosynthesis of a phytoalexin, {beta}-thujaplicin, were investigated in Cupressus lusitanica cell cultures. Yeast elicitor, methyl jasmonate, and ethylene all induce the production of {beta}-thujaplicin. Elicitor also stimulates the biosynthesis of jasmonate and ethylene before the induction of {beta}-thujaplicin accumulation. The elicitor-induced {beta}-thujaplicin accumulation can be partly blocked by inhibitors of jasmonate and ethylene biosynthesis or signal transduction. These results indicate that the jasmonate and ethylene signalling pathways are integral parts of the elicitor signal transduction leading to {beta}-thujaplicin accumulation. Methyl jasmonate treatment can induce ethylene production, whereas ethylene does not induce jasmonate biosynthesis; methyl jasmonate-induced {beta}-thujaplicin accumulation can be partly blocked by inhibitors of ethylene biosynthesis and signalling, while blocking jasmonate biosynthesis inhibits almost all ethylene-induced {beta}-thujaplicin accumulation. These results indicate that the ethylene and jasmonate pathways interact in mediating {beta}-thujaplicin production, with the jasmonate pathway working as a main control and the ethylene pathway as a fine modulator for {beta}-thujaplicin accumulation. Both the ethylene and jasmonate signalling pathways can be regulated upstream by Ca2+. Ca2+ influx negatively regulates ethylene production, and differentially regulates elicitor- or methyl jasmonate-stimulated ethylene production.

Key words: Calcium, Cupressus lusitanica, elicitor, ethylene, jasmonate, phytoalexin, signal transduction.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J Exp BotHome page
L. Almagro, L. V. Gomez Ros, S. Belchi-Navarro, R. Bru, A. Ros Barcelo, and M. A. Pedreno
Class III peroxidases in plant defence reactions
J. Exp. Bot., February 1, 2009; 60(2): 377 - 390.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
S.-J. Wu, J.-L. Qi, W.-J. Zhang, S.-H. Liu, F.-H. Xiao, M.-S. Zhang, G.-H. Xu, W.-G. Zhao, M.-W. Shi, Y.-J. Pang, et al.
Nitric Oxide Regulates Shikonin Formation in Suspension-Cultured Onosma paniculatum Cells
Plant Cell Physiol., January 1, 2009; 50(1): 118 - 128.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.