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JXB Advance Access published online on April 8, 2004
Journal of Experimental Botany, doi:10.1093/jxb/erh135
© 2004 by Oxford University Press
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Received September 15, 2003; accepted February 24, 2004
© 2004 Society for Experimental Biology

Article

Seeing ‘cool’ and ‘hot’--infrared thermography as a tool for non-invasive, high-throughput screening of Arabidopsis guard cell signalling mutants

Yibing Wang 1*, Geoffrey Holroyd 2, Alistair M. Hetherington 2, and Carl K-Y. Ng 3

1 Department of Biological Sciences, Institute of Environmental and Natural Sciences, Lancaster University, Bailrigg, Lancaster LA1 4YQ, UK; Department of Plant Sciences, University of Oxford, South Parks Road, Oxford OX1 3RB, UK
2 Department of Biological Sciences, Institute of Environmental and Natural Sciences, Lancaster University, Bailrigg, Lancaster LA1 4YQ, UK
3 Department of Botany, University College Dublin, Belfield, Dublin 4, Ireland

* To whom correspondence should be addressed. E-mail: carl.ng{at}ucd.ie.


  Abstract

The use of Arabidopsis mutants defective in abscisic acid (ABA) perception has been instrumental in the understanding of stomatal function, in particular, ABA signalling in guard cells. The considerable attention devoted to ABA signalling in guard cells is due in part to (1) the fundamental role of ABA in drought stress and (2) the use of a screening protocol based on the sensitivity of seed germination to ABA. Such a screen has facilitated the isolation of ABA signalling mutants with genetic lesions that exert pleiotropic effects at the whole plant level. As such, there is a requirement for new approaches to complement the seed germination screen. The recent advances made in the use of infrared thermography as a non-invasive, high-throughput tool are reviewed here and the versatility of this technique for screening Arabidopsis defective in stomatal regulation is highlighted.

Key words: Arabidopsis, drought, EMS mutants, infrared thermography, low humidity.


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