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JXB Advance Access published online on May 7, 2004
Journal of Experimental Botany, doi:10.1093/jxb/erh144
© 2004 by Oxford University Press
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Received December 9, 2003; accepted March 2, 2004
© 2004 Society for Experimental Biology

RESEARCH PAPER

Isolation of cucumber CsARF cDNAs and expression of the corresponding mRNAs during gravity-regulated morphogenesis of cucumber seedlings

Yuko Saito 1, Seiji Yamasaki 2, Nobuharu Fujii 1*, Gretchen Hagen 3, Tom Guilfoyle 3, and Hideyuki Takahashi 1

1 Graduate School of Life Sciences, Tohoku University, 2-1-1 Katahira, Aoba-ku, Sendai 980-8577, Japan
2 Graduate School of Life Sciences, Tohoku University, 2-1-1 Katahira, Aoba-ku, Sendai 980-8577, Japan; Fukuoka University of Education, 1-1 Akamabunkyomachi, Munakata, Fukuoka 811-4192, Japan
3 Department of Biochemistry, University of Missouri, 117 Schweitzer Hall, Columbia, Missouri 65211, USA

* To whom correspondence should be addressed. E-mail: nobuharu{at}ige.tohoku.ac.jp.


  Abstract

Cucumber seedlings show positive gravitropism and bend in the transition zone between the hypocotyl and the root. The peg, a specialized protuberance, develops on the concave side of the bending transition zone. Auxin and the mRNA of an auxin-inducible gene (CsIAA1) isolated from cucumber are differentially accumulated across the transition zone during the gravity-regulated peg formation. In this study, five cDNAs of Auxin Response Factors (ARFs) from cucumber were isolated and their mRNA accumulation was compared with that of CsIAA1. The tissue specificity of CsARF2 mRNA accumulation was similar to that of CsIAA1. Because the structural character of CsARF2 predicts that it is a transcriptional activator, CsARF2 may be involved in the activation of CsIAA1 transcription, which plays a role in gravity-regulated peg formation. Neither gravity nor auxin affected mRNA accumulation of five CsARFs including CsARF2, suggesting that CsARF2 may be regulated at a post-transcriptional level to induce the asymmetric expression of the CsIAA1 gene in response to gravistimulation and auxin in cucumber seedlings.

Key words: Aux/IAA, auxin response factors (ARFs), Cucumis sativus, gravity, peg.


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