Skip Navigation



JXB Advance Access published online on June 4, 2004
Journal of Experimental Botany, doi:10.1093/jxb/erh158
© 2004 by Oxford University Press
This Article
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
55/402/1463    most recent
erh158v1
Right arrow E-letters: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when E-letters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Zakharov, A.
Right arrow Articles by Saalbach, I.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zakharov, A.
Right arrow Articles by Saalbach, I.
Agricola
Right arrow Articles by Zakharov, A.
Right arrow Articles by Saalbach, I.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Received January 28, 2004; accepted March 18, 2004
© 2004 Society for Experimental Biology

RESEARCH PAPER

Seed-specific promoters direct gene expression in non-seed tissue

Alexander Zakharov 1, Martin Giersberg 2, Felicia Hosein 2, Michael Melzer 2, Klaus Müntz 2*, and Isolde Saalbach 2

1 Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK), Corrensstr. 3, D-06466 Gatersleben, Germany; Laboratory of Protein Chemistry, Moldova State University, Mateevici str. 60, MD-2009 Chisinau, Republic of Moldova
2 Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK), Corrensstr. 3, D-06466 Gatersleben, Germany

* To whom correspondence should be addressed. E-mail: muentz{at}IPK-Gatersleben.de.


  Abstract

The organ specificity of four promoters that are known to direct seed-specific gene expression was tested. Whereas the phaseolin (phas)- and legumin B4 (leB4)-promoters were from genes encoding 7S and 11S globulins from Phaseolus vulgaris and Vicia faba, respectively, the usp- and the sbp-promoters were from non-storage protein genes of V. faba. The expression of different promoter-reporter gene fusions was followed either by RT-PCR or by registering the reporter enzyme activity in organs of transgenic tobacco, pea, narbon bean, or linseed. In addition to seeds, the promoters directed reporter gene expression in pollen and in seed coats. USP-, vicilin- and legumin-mRNA were detected by RT-PCR in pollen of Pisum sativum and V. faba. Expression during microsporogenesis and embryogenesis seems to be a general character of various seed protein genes.

Key words: Gene expression, organ specificity, pollen, promoters, seed, seed coat.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Plant Physiol.Home page
A. Olbrich, S. Hillmer, G. Hinz, P. Oliviusson, and D. G. Robinson
Newly Formed Vacuoles in Root Meristems of Barley and Pea Seedlings Have Characteristics of Both Protein Storage and Lytic Vacuoles
Plant Physiology, December 1, 2007; 145(4): 1383 - 1394.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
R. Radchuk, V. Radchuk, W. Weschke, L. Borisjuk, and H. Weber
Repressing the Expression of the SUCROSE NONFERMENTING-1-RELATED PROTEIN KINASE Gene in Pea Embryo Causes Pleiotropic Defects of Maturation Similar to an Abscisic Acid-Insensitive Phenotype
Plant Physiology, January 1, 2006; 140(1): 263 - 278.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
A. Zakharov and K. Muntz
Seed legumains are expressed in stamens and vegetative legumains in seeds of Nicotiana tabacum L.
J. Exp. Bot., July 1, 2004; 55(402): 1593 - 1595.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.