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JXB Advance Access published online on July 2, 2004
Journal of Experimental Botany, doi:10.1093/jxb/erh186
© 2004 by Oxford University Press
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Received February 10, 2004
Accepted April 27, 2004

RESEARCH PAPER

Molecular characterization and expression studies during melon fruit development and ripening of L-galactono-1,4-lactone dehydrogenase

Irene Pateraki 1, Maite Sanmartin 2, Mary S. Kalamaki 3, Dimitrios Gerasopoulos 4, Angelos K. Kanellis 1*

1 Group of Biotechnology of Pharmaceutical Plants, Laboratory of Pharmacognocy, Department of Pharmaceutical Sciences, Aristotle University of Thessaloniki, 541 24 Thessaloniki, Greece; Institute of Viticulture and Vegetable Crops, National Agriculture Research Foundation, PO Box 2229, 713 09 Heraklion, Crete, Greece
2 Group of Biotechnology of Pharmaceutical Plants, Laboratory of Pharmacognocy, Department of Pharmaceutical Sciences, Aristotle University of Thessaloniki, 541 24 Thessaloniki, Greece; Institute of Viticulture and Vegetable Crops, National Agriculture Research Foundation, PO Box 2229, 713 09 Heraklion, Crete, Greece; Institute of Molecular Biology and Biotechnology, FORTH, PO Box 1527, 711 10 Heraklion, Crete, Greece; Present address: Departamento de Genética Molecular de Plantas, Centro Nacional de Biotecnología CSIC, 28049 Madrid, Spain
3 Group of Biotechnology of Pharmaceutical Plants, Laboratory of Pharmacognocy, Department of Pharmaceutical Sciences, Aristotle University of Thessaloniki, 541 24 Thessaloniki, Greece
4 Group of Biotechnology of Pharmaceutical Plants, Laboratory of Pharmacognocy, Department of Pharmaceutical Sciences, Aristotle University of Thessaloniki, 541 24 Thessaloniki, Greece; Present address: Department of Agriculture, Aristotle University of Thessaloniki, 541 24 Thessaloniki, Greece

* To whom correspondence should be addressed. E-mail: kanellis{at}pharm.auth.gr.


  Abstract

The last step of ascorbic acid (AA) biosynthesis is catalysed by the enzyme L-galactono-1,4-lactone dehydrogenase (GalLDH, EC 1.3.2.3), located on the inner mitochondrial membrane. The enzyme converts L-galactono-1,4-lactone to ascorbic acid (AA). In this work, the cloning and characterization of a GalLDH full-length cDNA from melon (Cucumis melo L.) are described. Melon genomic DNA Southern analysis indicated that CmGalLDH was encoded by a single gene. CmGalLDH mRNA accumulation was detected in all tissues studied, but differentially expressed during fruit development and seed germination. It is hypothesized that induction of CmGalLDH gene expression in ripening melon fruit contributes to parallel increases in the AA content and so playing a role in the oxidative ripening process. Higher CmGalLDH message abundance in light-grown seedlings compared with those raised in the dark suggests that CmGalLDH expression is regulated by light. Finally, various stresses and growth regulators resulted in no significant change in steady state levels of CmGalLDH mRNA in 20-d-old melon seedlings. To the authors' knowledge, this is the first report of GalLDH transcript induction in seed germination and differential gene expression during fruit ripening.

Keywords: Ascorbic acid; biosynthesis; Cucumis melo L.; fruit development; L-galactono-1,4-lactone dehydrogenase; melon; ripening.
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