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JXB Advance Access published online on July 30, 2004
Journal of Experimental Botany, doi:10.1093/jxb/erh217
© 2004 by Oxford University Press
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Received February 18, 2004
Accepted May 28, 2004

RESEARCH PAPER

The gene geranylgeranyl reductase of peach (Prunus persica [L.] Batsch) is regulated during leaf development and responds differentially to distinct stress factors

Donato Giannino 1*, Emiliano Condello 2, Leonardo Bruno 2, Giulio Testone 1, Andrea Tartarini 1, Radiana Cozza 2, Anna Maria Innocenti 2, Maria Beatrice Bitonti 2, Domenico Mariotti 1

1 Institute of Biology and Agricultural Biotechnology-Section of Rome, National Research Council of Italy (CNR), via Salaria km 29,300, I-00016, Monterotondo Scalo, Rome, Italy
2 Dipartimento di Ecologia dell'Università della Calabria, Ponte Bucci, I-87030, Arcavacata di Rende, Cosenza, Italy

* To whom correspondence should be addressed. E-mail: giannino{at}mlib.cnr.it.


  Abstract

Plant geranylgeranyl hydrogenase (CHL P) reduces free geranylgeranyl diphosphate to phytil diphosphate, which provides the side chain to chlorophylls, tocopherols, and plastoquinones. In peach, the single copy gene (PpCHL P) encodes a deduced product of 51.68 kDa, which harbours a transit peptide for cytoplasm-to-chloroplast transport and a nicotinamide binding domain. The PpCHL P message was abundant in chlorophyll-containing tissues and flower organs, but barely detected in the roots and mesocarp of ripening fruits, suggesting that transcription was related to plastid types and maturation. The message was not revealed in shoot apical meristems, but spread thoroughly in leaf cells during the early stages and was located mainly in the palisade of mature leaves, which exhibited higher transcript levels than young ones. Hence, the transcription of PpCHL P was likely to be regulated during leaf development. Gene expression was monitored in leaves responding to natural dark, cold, wounding, stress by imposed darkening, and during the curl disease. Transcription was stimulated by light, but repressed by dark and cold stress. In darkened leaves, the PpCHL P message was augmented concomitantly with that of CATALASE. In wounded leaves, the message decreased, but recovered rapidly, whereas in curled leaves, a reduction in gene expression was related to leaf damage intensity. However, transcript signals increased locally both in cells mechanically wounded by a needle and in those naturally injured by the pathogenic fungus Taphrina deformans. These data suggest that PpCHL P expression was regulated by photosynthetic activity and was possibly involved in the defence response.

Keywords: Geranylgeranyl reductase; leaf development; peach; Prunus persica L.; regulation; stress response.
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