Skip Navigation



JXB Advance Access published online on July 30, 2004
Journal of Experimental Botany, doi:10.1093/jxb/erh225
© 2004 by Oxford University Press
This Article
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
55/405/2087    most recent
erh225v1
Right arrow E-letters: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when E-letters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Leboeuf, E.
Right arrow Articles by Lahaye, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Leboeuf, E.
Right arrow Articles by Lahaye, M.
Agricola
Right arrow Articles by Leboeuf, E.
Right arrow Articles by Lahaye, M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Received September 17, 2003
Accepted June 8, 2004

RESEARCH PAPER

Physico-chemical characteristics of cell walls from Arabidopsis thaliana microcalli showing different adhesion strengths

Edouard Leboeuf 1, Séverine Thoiron 2, Marc Lahaye 3*

1 INRA-Unité de Recherches sur les Polysaccharides, leurs Organisations et Interactions, BP 71627, F-44316 Nantes, France; University of Nantes, Groupe de Physiologie et Pathologie Végétales, Faculté des Sciences et Techniques, 2 rue de la Houssinière, BP 92208, F-44322, Nantes cedex 3, France
2 University of Nantes, Groupe de Physiologie et Pathologie Végétales, Faculté des Sciences et Techniques, 2 rue de la Houssinière, BP 92208, F-44322, Nantes cedex 3, France
3 INRA-Unité de Recherches sur les Polysaccharides, leurs Organisations et Interactions, BP 71627, F-44316 Nantes, France

* To whom correspondence should be addressed. E-mail: Lahaye{at}nantes.inra.fr.


  Abstract

Changes in the composition and structure of cell walls and extracellular polysaccharides (ECP) were studied during the growth of suspension-cultured Arabidopsis thaliana microcalli. Three growth phases, namely the cell division phase, the cell expansion phase, and the stationary phase, were distinguished and associated with a decreasing cell cluster adhesion strength. Degradation of the homogalacturonan pectic backbone and of linear pectic side chains (1,4)-{beta}-D-galactan were observed concomitantly with the cell expansion and stationary phases and the decrease in cell adhesion. Also, in the stationary phase, branched (1,5)-{alpha}-L-arabinans were linearized. The AGP content of the culture medium increased while it decreased in the cell wall during cell growth and as cell adhesion decreased. These data suggest that, in addition to homogalacturonan, pectic side chains and AGP are involved in plant cell development and particularly in cell-cell attachment.

Keywords: AGP; Arabidopsis thaliana; (1,5)-{alpha}-L-arabinans; cell adhesion; (1,4)-{beta}-D-galactans; homogalacturonan.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Mol PlantHome page
J. J. Ordaz-Ortiz, S. E. Marcus, and J. Paul Knox
Cell Wall Microstructure Analysis Implicates Hemicellulose Polysaccharides in Cell Adhesion in Tomato Fruit Pericarp Parenchyma
Mol Plant, September 1, 2009; 2(5): 910 - 921.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
A. A. Arsovski, T. M. Popma, G. W. Haughn, N. C. Carpita, M. C. McCann, and T. L. Western
AtBXL1 Encodes a Bifunctional {beta}-D-Xylosidase/{alpha}-L-Arabinofuranosidase Required for Pectic Arabinan Modification in Arabidopsis Mucilage Secretory Cells
Plant Physiology, July 1, 2009; 150(3): 1219 - 1234.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
R. A. Chavez Montes, P. Ranocha, Y. Martinez, Z. Minic, L. Jouanin, M. Marquis, L. Saulnier, L. M. Fulton, C. S. Cobbett, F. Bitton, et al.
Cell Wall Modifications in Arabidopsis Plants with Altered {alpha}-L-Arabinofuranosidase Activity
Plant Physiology, May 1, 2008; 147(1): 63 - 77.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
E. Leboeuf, F. Guillon, S. Thoiron, and M. Lahaye
Biochemical and immunohistochemical analysis of pectic polysaccharides in the cell walls of Arabidopsis mutant QUASIMODO 1 suspension-cultured cells: implications for cell adhesion
J. Exp. Bot., December 1, 2005; 56(422): 3171 - 3182.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.